Second-harmonic microscopy of unstained living cardiac myocytes: measurements of sarcomere length with 20-nm accuracy
Optics Letters, Vol. 29, Issue 17, pp. 2031-2033 (2004)
http://dx.doi.org/10.1364/OL.29.002031
Acrobat PDF (307 KB)
Abstract
We extend second-harmonic generation (SHG) microscopy to the measurement of sarcomere length in unstained living cardiac myocytes with 20-nm accuracy. We quantify individual sarcomere shortening in the presence of saxitoxin and find that it is in agreement with mechanical measurements of atrial tissue contracture. This functional application of SHG microscopy is generally applicable to quantify the physiological effects of drugs on contractile tissue. Our data also suggest that packed myosin heads in sarcomere thick filaments are responsible for the large second-harmonic endogenous signal in muscle tissue.
© 2004 Optical Society of America
OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy
(180.5810) Microscopy : Scanning microscopy
(190.0190) Nonlinear optics : Nonlinear optics
(190.4160) Nonlinear optics : Multiharmonic generation
Citation
Thierry Boulesteix, Emmanuel Beaurepaire, Martin-Pierre Sauviat, and Marie-Claire Schanne-Klein, "Second-harmonic microscopy of unstained living cardiac
myocytes: measurements of sarcomere length with 20-nm
accuracy," Opt. Lett. 29, 2031-2033 (2004)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-29-17-2031
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Direct Raman Imaging Techniques for Study of the Subcellular Distribution of a Drug (AO)
- Direct measurement of the voltage sensitivity of second-harmonic generation from a membrane dye in patch-clamped cells (OL)
- Structure sensitivity in third-harmonic generation microscopy (OL)
- Fast image analysis in polarization SHG microscopy. (OE)
- Recent advances in two-photon imaging: technology developments and biomedical applications (COL)
Related Conference Papers
- Direct Backward-Emitted Third-Harmonic Generation and its Application to Clinical Microscopy
- Dynamic Visualization of Mitochondria in Cardiomyocytes with Third Harmonic Generation Microscopy
- In vivo study of early development of vertebrate nervous system using noninvasive harmonics optical microscopy
- Circular polarized second harmonic microscopy
- Harmonic optical microscopy for noninvasive in vivo embryonic studies
« Previous Article | Next Article »
OSA is a member of 