A novel approach to reconstructing both the absorption and the scattering properties of a turbid medium simultaneously from steady-state broadband spectral measurements is presented that utilizes second-differential fitting to the water spectrum to estimate the optical path length in tissue. Theoretical and experimental evidence is provided to demonstrate the robust accuracy of the spectroscopy approach and reconstructed absorption images. The steady-state broadband CCD system has the potential to provide accurate chromophore imaging without the technological complexity of time- or frequency-domain systems.
© 2004 Optical Society of America
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(170.6960) Medical optics and biotechnology : Tomography
Heng Xu, Brian W. Pogue, Hamid Dehghani, Keith D. Paulsen, Roger Springett, and Jeff F. Dunn, "Absorption and scattering imaging of tissue with steady-state second-differential spectral-analysis tomography," Opt. Lett. 29, 2043-2045 (2004)