We report the development of a high-speed wide-field fluorescence-lifetime imaging (FLIM) system that provides fluorescence-lifetime images at rates of as many as 29 frames/s. A FLIM multiwell plate reader and a potentially portable FLIM endoscopic system operating at 355-nm excitation have been demonstrated.
© 2004 Optical Society of America
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6920) Medical optics and biotechnology : Time-resolved imaging
J. Requejo-Isidro, J. McGinty, I. Munro, D. S. Elson, N. P. Galletly, M. J. Lever, M. A. A. Neil, G. W. H. Stamp, P. M. W. French, P. A. Kellett, J. D. Hares, and A. K. L. Dymoke-Bradshaw, "High-speed wide-field time-gated endoscopic fluorescence-lifetime imaging," Opt. Lett. 29, 2249-2251 (2004)