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Optics Letters

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  • Vol. 29, Iss. 21 — Nov. 1, 2004
  • pp: 2521–2523

Miniaturized two-photon microscope based on a flexible coherent fiber bundle and a gradient-index lens objective

Werner Göbel, Jason N. D. Kerr, Axel Nimmerjahn, and Fritjof Helmchen  »View Author Affiliations


Optics Letters, Vol. 29, Issue 21, pp. 2521-2523 (2004)
http://dx.doi.org/10.1364/OL.29.002521


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Abstract

We present a miniature, flexible two-photon microscope consisting of a fused coherent optical fiber bundle with 30,000 cores and a gradient-index lens objective. The laser focus of a standard two-photon laser-scanning microscope was scanned over the entrance surface of the fiber bundle, resulting in sequential coupling into individual cores. Fluorescent light was detected through the fiber bundle. Micrometer-sized fluorescent beads and pollen grains were readily resolved. In addition, fluorescently labeled blood vessels were imaged through the fiber bundle in rat brain in vivo.

© 2004 Optical Society of America

OCIS Codes
(060.2310) Fiber optics and optical communications : Fiber optics
(110.2350) Imaging systems : Fiber optics imaging
(110.2760) Imaging systems : Gradient-index lenses
(180.2520) Microscopy : Fluorescence microscopy

Citation
Werner Göbel, Jason N. D. Kerr, Axel Nimmerjahn, and Fritjof Helmchen, "Miniaturized two-photon microscope based on a flexible coherent fiber bundle and a gradient-index lens objective," Opt. Lett. 29, 2521-2523 (2004)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-29-21-2521


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