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Optics Letters

Optics Letters


  • Vol. 29, Iss. 22 — Nov. 15, 2004
  • pp: 2611–2613

Measuring 0.1-nm motion in 1 ms in an optical microscope with differential back-focal-plane detection

Lora Nugent-Glandorf and Thomas T. Perkins  »View Author Affiliations

Optics Letters, Vol. 29, Issue 22, pp. 2611-2613 (2004)

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Back-focal-plane detection of micrometer-sized beads offers subnanometer resolution for single-molecule, optical trapping experiments. However, laser beam-pointing instability and mechanical drift of the microscope limit the resolution of optical-trapping experiments. By combining two infrared lasers with improved differential beam-pointing stability (<0.05 ┬Árad), we simultaneously measure and subtract the motion of the microscope stage, leading to a resolution of <0.1 nm in 1 ms and stability of 0.5 nm over 60 s. Repeated steps of 0.4 nm at 1 Hz are resolved with a signal-to-noise ratio of 25.

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(180.3170) Microscopy : Interference microscopy

Lora Nugent-Glandorf and Thomas T. Perkins, "Measuring 0.1-nm motion in 1 ms in an optical microscope with differential back-focal-plane detection," Opt. Lett. 29, 2611-2613 (2004)

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