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Optics Letters

Optics Letters


  • Vol. 29, Iss. 23 — Dec. 1, 2004
  • pp: 2701–2703

Simple approach to one-laser, broadband coherent anti-Stokes Raman scattering microscopy

Tak W. Kee and Marcus T. Cicerone  »View Author Affiliations

Optics Letters, Vol. 29, Issue 23, pp. 2701-2703 (2004)

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Coherent anti-Stokes Raman scattering (CARS) microscopy is emerging as a powerful method for imaging materials and biological systems, partly because of its noninvasiveness and selective chemical sensitivity. However, its full potential for species-selective imaging is limited by a restricted spectral bandwidth. Recent increases in bandwidth are promising but still are not sufficient for the level of robust component discrimination that would be needed in a chemically complex milieu found, for example, in intracellular and extracellular environments. We demonstrate a truly broadband CARS imaging instrument that we use to acquire hyperspectral images with vibrational spectra over a bandwidth of 2500 cm-1 with a resolution of 13 cm-1.

OCIS Codes
(110.4190) Imaging systems : Multiple imaging
(180.6900) Microscopy : Three-dimensional microscopy
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering

Tak W. Kee and Marcus T. Cicerone, "Simple approach to one-laser, broadband coherent anti-Stokes Raman scattering microscopy," Opt. Lett. 29, 2701-2703 (2004)

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