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Optics Letters

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  • Vol. 29, Iss. 24 — Dec. 15, 2004
  • pp: 2881–2883

Velocimetric third-harmonic generation microscopy: micrometer-scale quantification of morphogenetic movements in unstained embryos

Delphine Débarre, Willy Supatto, Emmanuel Farge, Bruno Moulia, Marie-Claire Schanne-Klein, and Emmanuel Beaurepaire  »View Author Affiliations


Optics Letters, Vol. 29, Issue 24, pp. 2881-2883 (2004)
http://dx.doi.org/10.1364/OL.29.002881


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Abstract

We demonstrate the association of third-harmonic generation (THG) microscopy and particle image velocimetry (PIV) analysis as a novel functional imaging technique for automated micrometer-scale characterization of morphogenetic movements in developing embryos. Using a combined two-photon-excited fluorescence and THG microscope, we characterize the optical properties of Drosophila embryos and show that sustained THG imaging does not perturb sensitive developmental dynamics. Velocimetric THG imaging provides a quantitative description of the dynamics of internal structures in unstained wild-type and mutant embryos.

© 2004 Optical Society of America

OCIS Codes
(170.1420) Medical optics and biotechnology : Biology
(170.7050) Medical optics and biotechnology : Turbid media
(180.0180) Microscopy : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
(190.4180) Nonlinear optics : Multiphoton processes

Citation
Delphine Débarre, Willy Supatto, Emmanuel Farge, Bruno Moulia, Marie-Claire Schanne-Klein, and Emmanuel Beaurepaire, "Velocimetric third-harmonic generation microscopy: micrometer-scale quantification of morphogenetic movements in unstained embryos," Opt. Lett. 29, 2881-2883 (2004)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-29-24-2881


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References

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