Two-photon microscopy is a powerful tool for imaging of cells or tissues. However, it presents the drawback of being a laser-scanning technique that involves a long acquisition time for fluorescence-lifetime imaging. Thus it is commonly limited to intensity images that give only indications of the location of fluorophores but do not identify the physicochemical properties and interactions between cells' components. To protect biological samples from experiments that are too long and to provide a more comprehensive spectroscopic tool we have developed a time-resolved multifocal multiphoton microscope. This setup allows us to speed up the acquisition while retaining the possibility of measuring both intensity and lifetime images of the sample.

© 2004 Optical Society of America

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

Related Journal Articles

• Multifocal multiphoton microscopy (OL)
• Resolution improvement by nonconfocal theta microscopy (OL)
• Single-molecule detection with a two-photon fluorescence microscope with fast-scanning capabilities and polarization sensitivity (OL)
• Imaging Properties in Two-Photon Excitation Microscopy and Effects of Refractive-Index Mismatch in Thick Specimens (AO)
• Whole-field optically sectioned fluorescence lifetime imaging (OL)

Related Conference Papers

• Nanosecond Laser Multiphoton Scanning Fluorescence Microscopy
• Improvements in multi-photon laser scanning microscopy using photonic crystal fibre
• Fluorescence Lifetime Imaging Using Cheap LEDs as Illumination: Application in Microchannels
• Intracellular oxygen sensing using a tunable UV-visible-NIR fluorescence lifetime imaging microscopy system
• Fluorescence lifetime imaging for biomedicine