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Optics Letters

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  • Vol. 29, Iss. 6 — Mar. 15, 2004
  • pp: 584–586

Time-resolved fluorescence anisotropy imaging applied to live cells

Klaus Suhling, Jan Siegel, Peter M. P. Lanigan, Sandrine Lévêque-Fort, Stephen E. D. Webb, David Phillips, Daniel M. Davis, and Paul M. W. French  »View Author Affiliations


Optics Letters, Vol. 29, Issue 6, pp. 584-586 (2004)
http://dx.doi.org/10.1364/OL.29.000584


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Abstract

We have developed a wide-field time-resolved imaging system to image quantitatively both the fluorescence lifetime and the rotational correlation time of a fluorophore. Using a polarization-resolved imager, we simultaneously image orthogonal polarization components of the fluorescence emission onto a time-gated intensified CCD. We demonstrate imaging of solvent viscosity variations through the rotational correlation time of fluorescein in a multiwell plate and apply this technique to probe the microviscosity in live cells.

© 2004 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(300.2530) Spectroscopy : Fluorescence, laser-induced
(300.6500) Spectroscopy : Spectroscopy, time-resolved

Citation
Klaus Suhling, Jan Siegel, Peter M. P. Lanigan, Sandrine Lévêque-Fort, Stephen E. D. Webb, David Phillips, Daniel M. Davis, and Paul M. W. French, "Time-resolved fluorescence anisotropy imaging applied to live cells," Opt. Lett. 29, 584-586 (2004)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-29-6-584


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