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Effect of detector size on optical resolution in phase contrast microscopes

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Abstract

We examine the effects of detector size on the imaging properties of a scanning phase contrast microscope. The lateral size of the point-spread function is found to be wider when a point detector is used than when a much larger detector is used. Combining this finding with the well-known difficulties involved in using a confocal microscope in transmission mode suggests that a phase contrast microscope employing a point detector will in general have inferior imaging properties compared with one employing a large detector.

© 2004 Optical Society of America

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