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  • Vol. 30, Iss. 10 — May. 15, 2005
  • pp: 1087–1089

Underwater cytometer for in situ measurement of marine phytoplankton by a technique combining laser-induced fluorescence and laser Doppler velocimetry

Xuzhu Wang, Robert K. Chan, and Amelia S. K. Cheng  »View Author Affiliations


Optics Letters, Vol. 30, Issue 10, pp. 1087-1089 (2005)
http://dx.doi.org/10.1364/OL.30.001087


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Abstract

We present a new type of flow cytometer that can operate underwater for a long time, as long as days, for measuring the size distribution, concentration, and biomass of marine phytoplankton. The major improvement of the instrument over existing techniques is the elimination of sample preparation, which is achieved with a laser Doppler crossed-beam arrangement for both defining a measurement volume and measuring the speed of the particle traversing it. By simultaneously sampling the laser-induced fluorescence signal and the Doppler signals, the technique can discriminate sizes of phytoplankton.

© 2005 Optical Society of America

OCIS Codes
(010.4450) Atmospheric and oceanic optics : Oceanic optics
(120.3940) Instrumentation, measurement, and metrology : Metrology

Citation
Xuzhu Wang, Robert K. Chan, and Amelia S. K. Cheng, "Underwater cytometer for in situ measurement of marine phytoplankton by a technique combining laser-induced fluorescence and laser Doppler velocimetry," Opt. Lett. 30, 1087-1089 (2005)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-30-10-1087


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References

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