A ball lens coupled fiber-optic probe design is described for depth-resolved measurements of the fluorescence and reflectance properties of epithelial tissue. A reflectance target, fluorescence targets, and a two-layer tissue phantom consisting of fluorescent microspheres suspended in collagen are used to characterize the performance of the probe. Localization of the signal to within 300 µm of the probe tip is observed by use of reflectance and fluorescence targets in air. Differential enhancement of the fluorescence signal from the top layer of the two-layer tissue phantom is observed.
© 2005 Optical Society of America
(060.2310) Fiber optics and optical communications : Fiber optics
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
Richard A. Schwarz, Dizem Arifler, Sung K. Chang, Ina Pavlova, Insiya A. Hussain, Vivian Mack, Bob Knight, Rebecca Richards-Kortum, and Ann M. Gillenwater, "Ball lens coupled fiber-optic probe for depth-resolved spectroscopy of epithelial tissue," Opt. Lett. 30, 1159-1161 (2005)