We present a new technique that produces en face tomographic images with a 10-µs acquisition time per image. The setup consists of an interference microscope with stroboscopic illumination provided by a xenon arc flash lamp (10-µs flashes at 15 Hz). The tomographic images are obtained from two phase-opposed interferometric images recorded simultaneously by two synchronized CCD cameras. Transverse resolution better than 1.0 µm is achieved by use of high-numerical-aperture microscope objectives. The short coherence length of the source yields an axial resolution of 0.9 µm. 3×3 pixel binning leads to a detection sensitivity of 71 dB. Our system is suitable for various applications, particularly in biology for in vivo cellular-level imaging.
© 2005 Optical Society of America
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.7160) Medical optics and biotechnology : Ultrafast technology
(180.3170) Microscopy : Interference microscopy
G. Moneron, A. C. Boccara, and A. Dubois, "Stroboscopic ultrahigh-resolution full-field optical coherence tomography," Opt. Lett. 30, 1351-1353 (2005)