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  • Vol. 30, Iss. 13 — Jul. 1, 2005
  • pp: 1689–1691

Portable in situ fluorescence cytometry of microscale cell-based assays

Daniel A. Tatosian, Michael L. Shuler, and Donghyun Kim  »View Author Affiliations


Optics Letters, Vol. 30, Issue 13, pp. 1689-1691 (2005)
http://dx.doi.org/10.1364/OL.30.001689


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Abstract

A portable fluorescence cytometric system has been developed for characterizing chemical concentration and cellular status in microscale cell culture analog (µCCA) devices. Based on discrete optical components, the system provides a modular platform for real-time image measurements applicable to a variety of cell-based microassays. As a feasibility study, we investigated the real-time dynamics of daunorubicin uptake with cultured mouse L-cells in a µCCA compartment. Time course results measured by the portable fluorescence cytometric system confirmed that in the µCCA devices daunorubicin accumulation is proportional to the liquid turnover rate.

© 2005 Optical Society of America

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.1530) Medical optics and biotechnology : Cell analysis
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging

Citation
Daniel A. Tatosian, Michael L. Shuler, and Donghyun Kim, "Portable in situ fluorescence cytometry of microscale cell-based assays," Opt. Lett. 30, 1689-1691 (2005)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-30-13-1689


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