A portable fluorescence cytometric system has been developed for characterizing chemical concentration and cellular status in microscale cell culture analog (µCCA) devices. Based on discrete optical components, the system provides a modular platform for real-time image measurements applicable to a variety of cell-based microassays. As a feasibility study, we investigated the real-time dynamics of daunorubicin uptake with cultured mouse L-cells in a µCCA compartment. Time course results measured by the portable fluorescence cytometric system confirmed that in the µCCA devices daunorubicin accumulation is proportional to the liquid turnover rate.
© 2005 Optical Society of America
(110.0110) Imaging systems : Imaging systems
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.1530) Medical optics and biotechnology : Cell analysis
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
Daniel A. Tatosian, Michael L. Shuler, and Donghyun Kim, "Portable in situ fluorescence cytometry of microscale cell-based assays," Opt. Lett. 30, 1689-1691 (2005)