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  • Vol. 30, Iss. 14 — Jul. 15, 2005
  • pp: 1800–1802

Raman sorting and identification of single living micro-organisms with optical tweezers

Changan Xie, De Chen, and Yong-qing Li  »View Author Affiliations


Optics Letters, Vol. 30, Issue 14, pp. 1800-1802 (2005)
http://dx.doi.org/10.1364/OL.30.001800


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Abstract

We report on a novel technique for sorting and identification of single biological cells and food-borne bacteria based on laser tweezers and Raman spectroscopy (LTRS). With this technique, biological cells of different physiological states in a sample chamber were identified by their Raman spectral signatures and then they were selectively manipulated into a clean collection chamber with optical tweezers through a microchannel. As an example, we sorted the live and dead yeast cells into the collection chamber and validated this with a standard staining technique. We also demonstrated that bacteria existing in spoiled foods could be discriminated from a variety of food particles based on their characteristic Raman spectra and then isolated with laser manipulation. This label-free LTRS sorting technique may find broad applications in microbiology and rapid examination of food-borne diseases.

© 2005 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.1530) Medical optics and biotechnology : Cell analysis
(170.5660) Medical optics and biotechnology : Raman spectroscopy

Citation
Changan Xie, De Chen, and Yong-qing Li, "Raman sorting and identification of single living micro-organisms with optical tweezers," Opt. Lett. 30, 1800-1802 (2005)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-30-14-1800


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