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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 30, Iss. 16 — Aug. 15, 2005
  • pp: 2134–2136

Structure sensitivity in third-harmonic generation microscopy

Delphine Débarre, Willy Supatto, and Emmanuel Beaurepaire  »View Author Affiliations


Optics Letters, Vol. 30, Issue 16, pp. 2134-2136 (2005)
http://dx.doi.org/10.1364/OL.30.002134


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Abstract

We characterize experimentally the influence of sample structure and beam focusing on signal level in third-harmonic generation (THG) microscopy. In the case of a homogeneous spherical sample, the dependence of the signal on the size of the sphere can be controlled by modifying the Rayleigh length of the excitation beam. More generally, the influence of excitation focusing on the signal depends on sample geometry, allowing one to highlight certain structures within a complex system. We illustrate this point by focusing-based contrast modulation in THG images of Drosophila embryos.

© 2005 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
(190.4180) Nonlinear optics : Multiphoton processes

Citation
Delphine Débarre, Willy Supatto, and Emmanuel Beaurepaire, "Structure sensitivity in third-harmonic generation microscopy," Opt. Lett. 30, 2134-2136 (2005)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-30-16-2134


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