We have developed a filterless multicolor detection unit for fluorescence correlation spectroscopy (FCS). This grating-based setup is continuously tunable for multicolor separation and is thus a powerful alternative to the classical cascade of dichroic mirrors and filters. Our tailored platform allows for accommodation of up to 15 detection channels covering the entire visible spectral range. As a proof of principle, we successfully demonstrate simultaneous FCS of four distinct fluorescent quantum dot species being mixed in aqueous solution. Grating-based detection allows for spectral high-resolution FCS in a stable and compact setup and is a feasible tool for quantitative investigation of complex biomolecular dynamics on a single molecule level.
© 2005 Optical Society of America
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(300.2530) Spectroscopy : Fluorescence, laser-induced
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence
Markus Burkhardt, Katrin G. Heinze, and Petra Schwille, "Four-color fluorescence correlation spectroscopy realized in a grating-based detection platform," Opt. Lett. 30, 2266-2268 (2005)