Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Transillumination spatially modulated illumination microscopy

Not Accessible

Your library or personal account may give you access

Abstract

The diagnostic utility of a conventional transillumination microscope, the most common imaging modality in clinical use today, is limited by the microscope’s resolution. It is, however, possible to achieve lateral resolution well beyond the classical limit by using laterally structured illumination in a wide-field, nonconfocal microscope. In this method, the spatially modulated illumination (SMI) makes high-resolution information that is normally inaccessible visible in the observed image. Previously presented SMI microscopy systems operated in epifluorescence mode. We describe the design, construction, and testing of a novel transillumination SMI microscope. As transillumination is necessary for most medical applications, such as histopathologic evaluation of biopsy tissue and chromosomal analysis, such a system should have a significant diagnostic effect.

© 2005 Optical Society of America

Full Article  |  PDF Article
More Like This
Subwavelength size determination by spatially modulated illumination virtual microscopy

Antonio Virgilio Failla, Antonio Cavallo, and Christoph Cremer
Appl. Opt. 41(31) 6651-6659 (2002)

Nanosizing of fluorescent objects by spatially modulated illumination microscopy

Antonio Virgilio Failla, Udo Spoeri, Benno Albrecht, Alexander Kroll, and Christoph Cremer
Appl. Opt. 41(34) 7275-7283 (2002)

Isotropic image in structured illumination microscopy patterned with a spatial light modulator

Bo-Jui Chang, Li-Jun Chou, Yun-Ching Chang, and Su-Yu Chiang
Opt. Express 17(17) 14710-14721 (2009)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (8)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved