We describe the creation and implementation of a near-field scanning solid immersion microscope that is specifically tailored for use in microfluidic systems. The microscope comprises a newly fabricated Weierstrass solid immersion lens (SIL), which is detached from its substrate and is free floating in the fluid, and a laser optical tweezer, which serves both as a trapping beam for alignment and positioning of the SIL and as a near-field scanning beam that images the sample through the SIL. A discussion of the SIL's fabrication method is presented along with experimental results that demonstrate the effectiveness of our microscope design.
© 2005 Optical Society of America
Aaron L. Birkbeck, Sanja Zlatanovic, Sadik C. Esener, and Mihrimah Ozkan, "Laser-tweezer-controlled solid immersion microscopy in microfluidic systems," Opt. Lett. 30, 2712-2714 (2005)