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Optics Letters

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  • Editor: Anthony J. Campillo
  • Vol. 30, Iss. 20 — Oct. 15, 2005
  • pp: 2760–2762

Spectral- and frequency-encoded fluorescence imaging

Jason T. Motz, Dvir Yelin, Benjamin J. Vakoc, Brett E. Bouma, and Guillermo J. Tearney  »View Author Affiliations


Optics Letters, Vol. 30, Issue 20, pp. 2760-2762 (2005)
http://dx.doi.org/10.1364/OL.30.002760


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Abstract

A method for obtaining fluorescence images with a high number of resolvable points by using spectral and frequency encoding is presented. Broadband excitation light is encoded with a wavelength-dependent frequency modulation and dispersed onto the sample with a grating to simultaneously illuminate an entire image line. The Fourier transform of the frequency-encoded fluorescence emission provides one line of the image. Mechanical scanning along a direction orthogonal to the wavelength-encoded axis allows creation of the two-dimensional fluorescent image. This method is applicable for developing submillimeter diameter endoscopes. The principles of the technique are validated by imaging indocyanine green fluorescence in microfluidic channels.

© 2005 Optical Society of America

OCIS Codes
(110.2970) Imaging systems : Image detection systems
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence

ToC Category:
Medical Optics and Biotechnology

Citation
Jason T. Motz, Dvir Yelin, Benjamin J. Vakoc, Brett E. Bouma, and Guillermo J. Tearney, "Spectral- and frequency-encoded fluorescence imaging," Opt. Lett. 30, 2760-2762 (2005)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-30-20-2760


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