A method for obtaining fluorescence images with a high number of resolvable points by using spectral and frequency encoding is presented. Broadband excitation light is encoded with a wavelength-dependent frequency modulation and dispersed onto the sample with a grating to simultaneously illuminate an entire image line. The Fourier transform of the frequency-encoded fluorescence emission provides one line of the image. Mechanical scanning along a direction orthogonal to the wavelength-encoded axis allows creation of the two-dimensional fluorescent image. This method is applicable for developing submillimeter diameter endoscopes. The principles of the technique are validated by imaging indocyanine green fluorescence in microfluidic channels.
© 2005 Optical Society of America
(110.2970) Imaging systems : Image detection systems
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
Medical Optics and Biotechnology
Jason T. Motz, Dvir Yelin, Benjamin J. Vakoc, Brett E. Bouma, and Guillermo J. Tearney, "Spectral- and frequency-encoded fluorescence imaging," Opt. Lett. 30, 2760-2762 (2005)