We present a simple modification to a conventional wide-field fluorescence microscope that provides depth discrimination in thick tissues. The technique consists of illuminating a sample with a sequence of independent speckle patterns and displaying the rms of the resultant sequence of fluorescence images. The advantage of speckle illumination is that it provides diffraction-limited illumination granularity that is highly contrasted even in scattering media. We demonstrate quasi-confocal imaging in a mouse olfactory bulb labeled with green fluorescent protein.
© 2005 Optical Society of America
Vol. 1, Iss. 1 Virtual Journal for Biomedical Optics
Cathie Ventalon and Jerome Mertz, "Quasi-confocal fluorescence sectioning with dynamic speckle illumination," Opt. Lett. 30, 3350-3352 (2005)