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Optics Letters

Optics Letters


  • Editor: Anthony J. Campillo
  • Vol. 30, Iss. 24 — Dec. 15, 2005
  • pp: 3350–3352

Quasi-confocal fluorescence sectioning with dynamic speckle illumination

Cathie Ventalon and Jerome Mertz  »View Author Affiliations

Optics Letters, Vol. 30, Issue 24, pp. 3350-3352 (2005)

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We present a simple modification to a conventional wide-field fluorescence microscope that provides depth discrimination in thick tissues. The technique consists of illuminating a sample with a sequence of independent speckle patterns and displaying the rms of the resultant sequence of fluorescence images. The advantage of speckle illumination is that it provides diffraction-limited illumination granularity that is highly contrasted even in scattering media. We demonstrate quasi-confocal imaging in a mouse olfactory bulb labeled with green fluorescent protein.

© 2005 Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Virtual Issues
Vol. 1, Iss. 1 Virtual Journal for Biomedical Optics

Cathie Ventalon and Jerome Mertz, "Quasi-confocal fluorescence sectioning with dynamic speckle illumination," Opt. Lett. 30, 3350-3352 (2005)

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