We demonstrate an optically sectioned fluorescence lifetime imaging microscope with a wide-field detector, using a convenient, continuously tunable (435-1150 nm) ultrafast source for fluorescence imaging applications that is derived from a visible supercontinuum generated in a microstructured fiber.
© 2005 Optical Society of America
Vol. 1, Iss. 1 Virtual Journal for Biomedical Optics
D. M. Grant, D. S. Elson, D. Schimpf, C. Dunsby, J. Requejo-Isidro, E. Auksorius, I. Munro, M. A. Neil, P. M. French, E. Nye, G. Stamp, and P. Courtney, "Optically sectioned fluorescence lifetime imaging using a Nipkow disk microscope and a tunable ultrafast continuum excitation source," Opt. Lett. 30, 3353-3355 (2005)