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Optics Letters

Optics Letters


  • Vol. 30, Iss. 4 — Feb. 15, 2005
  • pp: 385–387

Use of a single-mode fiber coupler for second-harmonic-generation microscopy

Ling Fu, Xiaosong Gan, and Min Gu  »View Author Affiliations

Optics Letters, Vol. 30, Issue 4, pp. 385-387 (2005)

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We present a compact second-harmonic-generation (SHG) microscope based on a three-port single-mode fiber coupler. The fiber coupler is used to deliver a near-infrared ultrashort-pulsed laser beam as well as to collect the SHG signal in the visible wavelength range. The SHG microscope exhibits an axial resolution of 1.8 µm, which shows a slight enhancement of the optical sectioning effect compared with that for two-photon excitation at the same excitation wavelength. It is also demonstrated that SHG and two-photon fluorescence images under parallel and perpendicular laser excitation polarization can be simultaneously obtained.

© 2005 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.2350) Imaging systems : Fiber optics imaging
(180.2520) Microscopy : Fluorescence microscopy

Ling Fu, Xiaosong Gan, and Min Gu, "Use of a single-mode fiber coupler for second-harmonic-generation microscopy," Opt. Lett. 30, 385-387 (2005)

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  1. Y. Guo, P. P. Ho, H. Savage, D. Harris, P. Sacks, S. Schantz, F. Liu, N. Zhadin, and R. R. Alfano, Opt. Lett. 22, 1323 (1997).
  2. P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, Biophys. J. 81, 493 (2002).
  3. A. Zoumi, A. Yeh, and B. J. Tromberg, Proc. Natl. Acad. Sci. USA 99 11,014 (2002).
  4. D. A. Dombeck, K. A. Kasischke, H. D. Vishwasrao, M. Ingelsson, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100 7081 (2003).
  5. W. E. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100 7075 (2003).
  6. P. J. Campagnola and L. M. Loew, Nat. Biotechnol. 21, 1356 (2003).
  7. D. Bird and M. Gu, Opt. Lett. 27, 1031 (2002).
  8. D. Bird and M. Gu, Opt. Lett. 28, 1552 (2003).
  9. D. Bird and M. Gu, Appl. Opt. 41, 1852 (2002).
  10. D. Bird and M. Gu, J. Microsc. (Oxford) 208, 35 (2002).
  11. G. P. Agrawal, Nonlinear Fiber Optics, (Academic, San Diego, Calif., 1989).
  12. P. J. Campagnola, M. Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3341 (1999).
  13. L. Liu, X. Deng, L. Yang, G. Wang, and Z. Xu, Opt. Lett. 25, 1711 (2000).
  14. L. Fu, X. Gan, D. Bird, and M. Gu, "Polarisation characteristics of a 1×2 fibre coupler under femtosecond pulsed and continuous wave illumination" Opt. Laser Technol. (to be published).

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