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Optics Letters

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  • Vol. 30, Iss. 4 — Feb. 15, 2005
  • pp: 385–387

Use of a single-mode fiber coupler for second-harmonic-generation microscopy

Ling Fu, Xiaosong Gan, and Min Gu  »View Author Affiliations


Optics Letters, Vol. 30, Issue 4, pp. 385-387 (2005)
http://dx.doi.org/10.1364/OL.30.000385


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Abstract

We present a compact second-harmonic-generation (SHG) microscope based on a three-port single-mode fiber coupler. The fiber coupler is used to deliver a near-infrared ultrashort-pulsed laser beam as well as to collect the SHG signal in the visible wavelength range. The SHG microscope exhibits an axial resolution of 1.8 µm, which shows a slight enhancement of the optical sectioning effect compared with that for two-photon excitation at the same excitation wavelength. It is also demonstrated that SHG and two-photon fluorescence images under parallel and perpendicular laser excitation polarization can be simultaneously obtained.

© 2005 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.2350) Imaging systems : Fiber optics imaging
(180.2520) Microscopy : Fluorescence microscopy

Citation
Ling Fu, Xiaosong Gan, and Min Gu, "Use of a single-mode fiber coupler for second-harmonic-generation microscopy," Opt. Lett. 30, 385-387 (2005)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-30-4-385


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