OSA's Digital Library

Optics Letters

Optics Letters


  • Vol. 30, Iss. 6 — Mar. 15, 2005
  • pp: 625–627

In vivo dark-field reflection-mode photoacoustic microscopy

Konstantin Maslov, Gheorghe Stoica, and Lihong V. Wang  »View Author Affiliations

Optics Letters, Vol. 30, Issue 6, pp. 625-627 (2005)

View Full Text Article

Acrobat PDF (335 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



Reflection-mode photoacoustic microscopy with dark-field laser pulse illumination and high-numerical-aperture ultrasonic detection is designed and implemented in noninvasively imaged blood vessels in the skin in vivo. Dark-field optical illumination minimizes the interference caused by strong photoacoustic signals from superficial structures. A high-numerical-aperture acoustic lens provides high lateral resolution, 45-120 µm in this system. A broadband ultrasonic detection system provides high axial resolution, estimated to be ∼15 µm. The optical illumination and ultrasonic detection are in a coaxial confocal configuration for optimal image quality. The system is capable of imaging optical-absorption contrast as deep as 3 mm in biological tissue.

© 2005 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(180.5810) Microscopy : Scanning microscopy

Konstantin Maslov, Gheorghe Stoica, and Lihong V. Wang, "In vivo dark-field reflection-mode photoacoustic microscopy," Opt. Lett. 30, 625-627 (2005)

Sort:  Author  |  Year  |  Journal  |  Reset


  1. H. Nakajima, T. Minabe, and N. Imanishi, Plast. Reconstr. Surg. 102, 748 (1998).
  2. P. Carmeliet and R. K. Jain, Nature (London) 407, 6801 (2000). [CrossRef]
  3. S. J. Nelson and S. Cha, Cancer J. Sci. Am. 9, 134 (2003).
  4. X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotechnol. 21, 803 (2003). [CrossRef]
  5. M. Xu and L. V. Wang, IEEE Trans. Biomed. Eng. 50, 1086 (2002). [CrossRef]
  6. A. A. Oraevsky, V. A. Andreev, A. A. Karabutov, D. R. Fleming, Z. Gatalica, H. Singh, and R. O. Esenaliev, Proc. SPIE 3597, 352 (1999).
  7. C. G. A. Hoelen, F. F. M. de Mul, R. Pongers, and A. Dekker, Opt. Lett. 23, 648 (1998).
  8. R. G. M. Kolkman, E. Hondebrink, W. Steenbergen, and F. F. M. de Mul, IEEE J. Sel. Top. Quantum Electron. 9, 343 (2003). [CrossRef]
  9. C. Guittet, F. Ossant, L. Vaillant, and M. Berson, IEEE Trans. Biomed. Eng. 46, 740 (1999).
  10. American National Standards Institute, "American National Standard for the Safe Use of Lasers," ANSI Standard Z136.1 (American National Standards Institute, New York, 2000).
  11. S. T. Flock, S. L. Jacques, B. C. Wilson, W. M. Star, and M. J. C. van Gemert, Lasers Surg. Med. 12, 510 (1992).
  12. W. F. Cheong, S. A. Prahl, and A. J. Welch, IEEE J. Quantum Electron. 26, 2166 (1990).
  13. W. J. Smith, Modern Optical Engineering, (McGraw-Hill, New York, 1966), p. 318.
  14. National Institutes of Health, "Guide for the care and use of laboratory animals, revised ed.,(( NIH Publ. 86-23 (U.S. Government Printing Office, Washington, D.C., 1985).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited