We report on the applicability of combining surface-enhanced Raman scattering (SERS) with coherent anti-Stokes Raman scattering for high-sensitivity detection of biological molecules. We found that this combination of techniques provides more than 3 orders of signal enhancement compared with SERS and permits monitoring of biological molecules such as deoxyguanosine monophosphate (dGMP) and deoxyadenosine monophosphate at the single-molecule level. This combined technique also improved detection sensitivity for angiotensin peptide. As this is believed to be the first report of detection of dGMP at the single-molecule level, we suggest that this approach can serve as a new tool for biological studies.
© 2005 Optical Society of America
(170.7160) Medical optics and biotechnology : Ultrafast technology
(240.4350) Optics at surfaces : Nonlinear optics at surfaces
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(320.0320) Ultrafast optics : Ultrafast optics
Tae-Woong Koo, Selena Chan, and Andrew A. Berlin, "Single-molecule detection of biomolecules by surface-enhanced coherent anti-Stokes Raman scattering," Opt. Lett. 30, 1024-1026 (2005)