OSA's Digital Library

Optics Letters

Optics Letters


  • Editor: Anthony J. Campillo
  • Vol. 31, Iss. 13 — Jul. 1, 2006
  • pp: 2015–2017

Diagnosis of colorectal cancer using Raman spectroscopy of laser-trapped single living epithelial cells

Kun Chen, Yejun Qin, Feng Zheng, Menghong Sun, and Daren Shi  »View Author Affiliations

Optics Letters, Vol. 31, Issue 13, pp. 2015-2017 (2006)

View Full Text Article

Enhanced HTML    Acrobat PDF (200 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



A single-cell diagnostic technique for epithelial cancers is developed by utilizing laser trapping and Raman spectroscopy to differentiate cancerous and normal epithelial cells. Single-cell suspensions were prepared from surgically removed human colorectal tissues following standard primary culture protocols and examined in a near-infrared laser-trapping Raman spectroscopy system, where living epithelial cells were investigated one by one. A diagnostic model was built on the spectral data obtained from 8 patients and validated by the data from 2 new patients. Our technique has potential applications from epithelial cancer diagnosis to the study of cell dynamics of carcinogenesis.

© 2006 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.4730) Medical optics and biotechnology : Optical pathology
(170.5660) Medical optics and biotechnology : Raman spectroscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 12, 2006
Manuscript Accepted: April 13, 2006

Virtual Issues
Vol. 1, Iss. 8 Virtual Journal for Biomedical Optics

Kun Chen, Yejun Qin, Feng Zheng, Menghong Sun, and Daren Shi, "Diagnosis of colorectal cancer using Raman spectroscopy of laser-trapped single living epithelial cells," Opt. Lett. 31, 2015-2017 (2006)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. Y. L. Kim, Y. Liu, R. K. Wali, H. K. Roy, M. J. Goldberg, A. K. Kromin, K. Chen, and V. Backman, IEEE J. Sel. Top. Quantum Electron. 9, 243 (2003). [CrossRef]
  2. Z. J. Smith and A. J. Berger, Opt. Lett. 30, 1363 (2005). [CrossRef] [PubMed]
  3. Y. Wu and J. Y. Qu, Opt. Lett. 30, 3045 (2005). [CrossRef] [PubMed]
  4. C. Xie, M. A. Dinno, and Y. Q. Li, Opt. Lett. 27, 249 (2002). [CrossRef]
  5. J. W. Chan, D. S. Taylor, T. Zwerdling, S. M. Lane, K. Ihara, and T. Huser, Biophys. J. 90, 648 (2006). [CrossRef]
  6. K. G. MacLeod and S. P. Langdon, Methods Mol. Biol. 88, 17 (2004).
  7. J. L. Deng, Q. Wei, M. H. Zhang, Y. Z. Wang, and Y. Q. Li, J. Raman Spectrosc. 36, 257 (2005). [CrossRef]
  8. G. Deinum, D. Rodriguez, T. J. Romer, M. Fitzmaurice, J. R. Kramer, and M. S. Feld, Appl. Spectrosc. 53, 938 (1999). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited