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Optics Letters

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  • Editor: Anthony J. Campillo
  • Vol. 31, Iss. 15 — Aug. 1, 2006
  • pp: 2308–2310

In vivo measurement of retinal physiology with high-speed ultrahigh-resolution optical coherence tomography

V. J. Srinivasan, M. Wojtkowski, J. G. Fujimoto, and J. S. Duker  »View Author Affiliations


Optics Letters, Vol. 31, Issue 15, pp. 2308-2310 (2006)
http://dx.doi.org/10.1364/OL.31.002308


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Abstract

Noninvasive in vivo functional optical imaging of the intact retina is demonstrated by using high-speed, ultrahigh-resolution optical coherence tomography (OCT). Imaging was performed with 2.8 μ m resolution at a rate of 24,000 axial scans per second. A white-light stimulus was applied to the dark-adapted rat retina, and the average reflectivities from different intraretinal layers were monitored as a function of time. A 10%–15% increase in the average amplitude reflectance of the photoreceptor outer segments was observed in response to the stimulus. The spatial distribution of the change in the OCT signal is consistent with an increase in backscatter from the photoreceptor outer segments. To our knowledge, this is the first in vivo demonstration of OCT functional imaging in the intact retina.

© 2006 Optical Society of America

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: March 8, 2006
Revised Manuscript: May 8, 2006
Manuscript Accepted: May 17, 2006
Published: July 10, 2006

Virtual Issues
Vol. 1, Iss. 9 Virtual Journal for Biomedical Optics

Citation
V. J. Srinivasan, M. Wojtkowski, J. G. Fujimoto, and J. S. Duker, "In vivo measurement of retinal physiology with high-speed ultrahigh-resolution optical coherence tomography," Opt. Lett. 31, 2308-2310 (2006)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-31-15-2308


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References

  1. A. Grinvald, R. D. Frostig, E. Lieke, and R. Hildesheim, Physiol. Rev. 68, 1285 (1988). [PubMed]
  2. A. Villringer and B. Chance, Trends Neurosci. 20, 435 (1997). [CrossRef] [PubMed]
  3. K. Tsunoda, Y. Oguchi, G. Hanazono, and M. Tanifuji, Invest. Ophthalmol. Visual Sci. 45, 3820 (2004). [CrossRef]
  4. D. A. Nelson, S. Krupsky, A. Pollack, E. Aloni, M. Belkin, I. Vanzetta, M. Rosner, and A. Grinvald, Invest. Ophthalmol. Visual Sci. 36, 57 (2005).
  5. M. D. Abramoff, Y. H. Kwon, D. Ts'o, P. Soliz, B. Zimmerman, J. Pokorny, and R. Kardon, Invest. Ophthalmol. Visual Sci. 47, 715 (2006). [CrossRef]
  6. M. Lazebnik, D. L. Marks, K. Potgieter, R. Gillette, and S. A. Boppart, Opt. Lett. 28, 1218 (2003). [CrossRef] [PubMed]
  7. R. U. Maheswari, H. Takaoka, H. Kadono, R. Homma, and M. Tanifuji, J. Neurosci. Methods 124, 83 (2003). [CrossRef] [PubMed]
  8. K. Bizheva, R. Pflug, B. Hermann, B. Povazay, H. Sattmann, P. Qiu, E. Anger, H. Reitsamer, S. Popov, J. R. Taylor, A. Unterhuber, P. Ahnelt, and W. Drexler, Proc. Natl. Acad. Sci. U.S.A. 103, 5066 (2006). [CrossRef] [PubMed]
  9. X. C. Yao, A. Yamauchi, B. Perry, and J. S. George, Appl. Opt. 44, 2019 (2005). [CrossRef] [PubMed]
  10. A. F. Fercher, C. K. Hitzenberger, G. Kamp, and S. Y. Elzaiat, Opt. Commun. 117, 43 (1995). [CrossRef]
  11. M. Wojtkowski, T. Bajraszewski, P. Targowski, and A. Kowalczyk, Opt. Lett. 28, 1745 (2003). [CrossRef] [PubMed]
  12. N. A. Nassif, B. Cense, B. H. Park, M. C. Pierce, S. H. Yun, B. E. Bouma, G. J. Tearney, T. C. Chen, and J. F. de Boer, Opt. Express 12, 367 (2004). [CrossRef] [PubMed]
  13. M. Wojtkowski, V. J. Srinivasan, T. H. Ko, J. G. Fujimoto, A. Kowalevicz, and J. S. Duker, Opt. Express 12, 2404 (2004). [CrossRef] [PubMed]
  14. R. Tripathi, N. Nassif, J. S. Nelson, B. H. Park, and J. F. de Boer, Opt. Lett. 27, 406 (2002). [CrossRef]
  15. P. J. DeLint, T. T. Berendschot, J. van de Kraats, and D. van Norren, Invest. Ophthalmol. Visual Sci. 41, 282 (2000).
  16. F. Uehara, M. T. Matthes, D. Yasumura, and M. M. Lavail, Science 248, 1633 (1990). [CrossRef] [PubMed]

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