Hybrid reflecting objectives for functional multiphoton microscopy in turbid media
Optics Letters, Vol. 31, Issue 16, pp. 2447-2449 (2006)
http://dx.doi.org/10.1364/OL.31.002447
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Abstract
Most multiphoton imaging of biological specimens is performed using microscope objectives optimized for high image quality under wide-field illumination. We present a class of objectives designed de novo without regard for these traditional constraints, driven exclusively by the needs of fast multiphoton imaging in turbid media: the delivery of femtosecond pulses without dispersion and the efficient collection of fluorescence. We model the performance of one such design optimized for a typical brain-imaging setup and show that it can greatly outperform objectives commonly used for this task.
© 2006 Optical Society of America
OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.7050) Medical optics and biotechnology : Turbid media
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: March 20, 2006
Revised Manuscript: May 23, 2006
Manuscript Accepted: May 29, 2006
Published: July 25, 2006
Virtual Issues
Vol. 1, Iss. 9 Virtual Journal for Biomedical Optics
Citation
Dejan Vucinic, Thomas M. Bartol, Jr., and Terrence J. Sejnowski, "Hybrid reflecting objectives for functional multiphoton microscopy in turbid media," Opt. Lett. 31, 2447-2449 (2006)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-31-16-2447
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