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Optics Letters

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  • Editor: Anthony J. Campillo
  • Vol. 31, Iss. 16 — Aug. 15, 2006
  • pp: 2447–2449

Hybrid reflecting objectives for functional multiphoton microscopy in turbid media

Dejan Vučinić, Thomas M. Bartol, Jr., and Terrence J. Sejnowski  »View Author Affiliations


Optics Letters, Vol. 31, Issue 16, pp. 2447-2449 (2006)
http://dx.doi.org/10.1364/OL.31.002447


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Abstract

Most multiphoton imaging of biological specimens is performed using microscope objectives optimized for high image quality under wide-field illumination. We present a class of objectives designed de novo without regard for these traditional constraints, driven exclusively by the needs of fast multiphoton imaging in turbid media: the delivery of femtosecond pulses without dispersion and the efficient collection of fluorescence. We model the performance of one such design optimized for a typical brain-imaging setup and show that it can greatly outperform objectives commonly used for this task.

© 2006 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.7050) Medical optics and biotechnology : Turbid media

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: March 20, 2006
Revised Manuscript: May 23, 2006
Manuscript Accepted: May 29, 2006
Published: July 25, 2006

Virtual Issues
Vol. 1, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Dejan Vucinic, Thomas M. Bartol, Jr., and Terrence J. Sejnowski, "Hybrid reflecting objectives for functional multiphoton microscopy in turbid media," Opt. Lett. 31, 2447-2449 (2006)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-31-16-2447


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