For what we believe to be the first time, digital holographic microscopy is applied to perform optical diffraction tomography of a pollen grain. Transmission phase images with nanometric axial accuracy are numerically reconstructed from holograms acquired for different orientations of the rotating sample; then the three-dimensional refractive index spatial distribution is computed by inverse radon transform. A precision of 0.01 for the refractive index estimation and a spatial resolution in the micrometer range are demonstrated.
© 2006 Optical Society of America
Vol. 1, Iss. 2 Virtual Journal for Biomedical Optics
Florian Charrière, Anca Marian, Frédéric Montfort, Jonas Kuehn, Tristan Colomb, Etienne Cuche, Pierre Marquet, and Christian Depeursinge, "Cell refractive index tomography by digital holographic microscopy," Opt. Lett. 31, 178-180 (2006)