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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Anthony J. Campillo
  • Vol. 31, Iss. 21 — Nov. 1, 2006
  • pp: 3119–3121

Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy

Chung-Chieh Yu, Condon Lau, James W. Tunnell, Martin Hunter, Maxim Kalashnikov, Christopher Fang-Yen, Stephen F. Fulghum, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld  »View Author Affiliations


Optics Letters, Vol. 31, Issue 21, pp. 3119-3121 (2006)
http://dx.doi.org/10.1364/OL.31.003119


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Abstract

We describe azimuthal light scattering spectroscopy ( ϕ LSS ) , a novel technique for assessing epithelial-cell nuclear morphology. The difference between the spectra measured at azimuthal angles ϕ = 0 ° and ϕ = 90 ° preferentially isolates the single backscattering contribution due to large ( 10 μ m ) structures such as epithelial cell nuclei by discriminating against scattering from smaller organelles and diffusive background. We demonstrate the feasibility of using ϕ LSS for cancer detection by showing that spectra from cancerous colon tissue exhibit significantly greater azimuthal asymmetry than spectra from normal colonic tissues.

© 2006 Optical Society of America

OCIS Codes
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(290.0290) Scattering : Scattering

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: June 30, 2006
Manuscript Accepted: July 21, 2006
Published: October 11, 2006

Virtual Issues
Vol. 1, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Chung-Chieh Yu, Condon Lau, James W. Tunnell, Martin Hunter, Maxim Kalashnikov, Christopher Fang-Yen, Stephen F. Fulghum, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld, "Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy," Opt. Lett. 31, 3119-3121 (2006)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-31-21-3119


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References

  1. H. Fang, M. Ollera, E. Vitkin, L. M. Kimerer, P. B. Cipolloni, M. M. Zaman, S. D. Freedman, I. J. Bigio, I. Itzkan, E. B. Hanlon, and L. T. Perelman, IEEE J. Sel. Top. Quantum Electron. 9, 267 (2003). [CrossRef]
  2. M. Bartlett, G. Huang, L. Larcom, and H. Jiang, Appl. Opt. 43, 1296 (2004). [CrossRef] [PubMed]
  3. J. D. Wilson, C. E. Bigelow, D. J. Calkins, and T. H. Foster, Biophys. J. 88, 2929 (2005). [CrossRef] [PubMed]
  4. J. D. Wilson and T. H. Foster, Opt. Lett. 30, 2442 (2005). [CrossRef] [PubMed]
  5. L. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. Crawford, and M. Feld, Phys. Rev. Lett. 80, 627 (1998). [CrossRef]
  6. V. Backman, R. Gurjar, K. Badizadegan, I. Itzkan, R. Dasari, L. Perelman, and M. Feld, IEEE J. Sel. Top. Quantum Electron. 5, 1019 (1999). [CrossRef]
  7. K. Sokolov, R. Drezek, K. Gossage, and R. Richards-Kortum, Opt. Express 5, 302 (1999). [CrossRef] [PubMed]
  8. J. Mourant, J. Freyer, A. Hielscher, A. Eick, D. Shen, and T. Johnson, Appl. Opt. 37, 3586 (1998). [CrossRef]
  9. H. C. van de Hulst, Light Scattering by Small Particles (Dover, New York, 1981).

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