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Optics Letters

Optics Letters


  • Editor: Anthony J. Campillo
  • Vol. 31, Iss. 24 — Dec. 15, 2006
  • pp: 3656–3658

Dual-pump coherent anti-Stokes–Raman scattering microscopy

Ondrej Burkacky, Andreas Zumbusch, Christian Brackmann, and Annika Enejder  »View Author Affiliations

Optics Letters, Vol. 31, Issue 24, pp. 3656-3658 (2006)

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We introduce dual-pump coherent anti-Stokes–Raman scattering (dual-CARS) microscopy. This new technique permits simultaneous imaging of two species characterized by different molecular vibrations, as well as the removal of nonresonant background. This is achieved by using three synchronized laser pulses probing two different vibrations. We demonstrate the virtues of the method by imaging a mixture of nondeuterated and deuterated lipids, clearly distinguishing the individual components and their organization in the mixed arrangement. Further, dual-CARS images of lipid stores in living Caenorhabditis elegans nematodes show that the suppression of the nonresonant background results in significantly enhanced image contrast.

© 2006 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.5810) Microscopy : Scanning microscopy
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering

ToC Category:

Original Manuscript: August 22, 2006
Revised Manuscript: September 28, 2006
Manuscript Accepted: September 28, 2006
Published: November 22, 2006

Virtual Issues
Vol. 2, Iss. 1 Virtual Journal for Biomedical Optics

Ondrej Burkacky, Andreas Zumbusch, Christian Brackmann, and Annika Enejder, "Dual-pump coherent anti-Stokes-Raman scattering microscopy," Opt. Lett. 31, 3656-3658 (2006)

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