We have developed diffraction phase microscopy as a new technique for quantitative phase imaging of biological structures. The method combines the principles of common path interferometry and single-shot phase imaging and is characterized by subnanometer path-length stability and millisecond-scale acquisition time. The potential of the technique for quantifying nanoscale motions in live cells is demonstrated by experiments on red blood cells.
© 2006 Optical Society of America
Medical Optics and Biotechnology
Original Manuscript: September 23, 2005
Revised Manuscript: November 12, 2005
Manuscript Accepted: December 5, 2005
Vol. 1, Iss. 4 Virtual Journal for Biomedical Optics
Gabriel Popescu, Takahiro Ikeda, Ramachandra R. Dasari, and Michael S. Feld, "Diffraction phase microscopy for quantifying cell structure and dynamics," Opt. Lett. 31, 775-777 (2006)