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Optics Letters

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  • Editor: Anthony J. Campillo
  • Vol. 32, Iss. 10 — May. 15, 2007
  • pp: 1308–1310

Total internal reflection ellipsometry: ultrahigh sensitivity for protein adsorption on metal surfaces

Michal Poksinski and Hans Arwin  »View Author Affiliations


Optics Letters, Vol. 32, Issue 10, pp. 1308-1310 (2007)
http://dx.doi.org/10.1364/OL.32.001308


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Abstract

Total internal reflection ellipsometry (TIRE) is used to study adsorption of human serum albumin and fibrinogen on thin gold films. TIRE shows very high sensitivity for protein monolayers adsorbed on metal surfaces when surface plasmon resonance effects are utilized. The measured data, expressed in ellipsometric angles ψ and Δ are of several orders of magnitude larger in comparison with those from similar experiments performed with traditional ellipsometry. TIRE in spectral mode opens a new path for precise studies of organic layers adsorbed on metal surfaces, with a potential for resolving the adsorbed layer microstructure.

© 2007 Optical Society of America

OCIS Codes
(120.2130) Instrumentation, measurement, and metrology : Ellipsometry and polarimetry
(160.4760) Materials : Optical properties
(160.4890) Materials : Organic materials
(240.0310) Optics at surfaces : Thin films
(240.6680) Optics at surfaces : Surface plasmons
(300.6550) Spectroscopy : Spectroscopy, visible

ToC Category:
Optics at Surfaces

History
Original Manuscript: November 10, 2006
Revised Manuscript: January 26, 2007
Manuscript Accepted: February 12, 2007
Published: April 17, 2007

Virtual Issues
Vol. 2, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Michal Poksinski and Hans Arwin, "Total internal reflection ellipsometry: ultrahigh sensitivity for protein adsorption on metal surfaces," Opt. Lett. 32, 1308-1310 (2007)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-32-10-1308


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References

  1. M. Poksinski and H. Arwin, are preparing a paper to be called "Total internal reflection ellipsometry--Design of experimental setup."
  2. H. Arwin, M. Poksinski, and K. Johansen, Appl. Opt. 43, 3028 (2004). [CrossRef] [PubMed]
  3. R. M. A. Azzam and N. M. Bashara, Ellipsometry and Polarized Light (North-Holland, 1987).
  4. H. G. Tompkins and E. A. Irene, eds., Handbook of Ellipsometry (Springer-Verlag, 2005). [CrossRef]
  5. S. Rekveld, Ellipsometric Studies of Protein Adsorption onto Hard Surfaces in a Flow Cell (Fedobruk, 1997).
  6. M. Poksinski, H. Dzuho, J.-O. Järrhed, and H. Arwin, in Eurosensors XIV, R.de Reus and S.Bouwstra, eds., (MIC, 2000), p. 239.
  7. P. Westphal and A. Bornmann, Sens. Actuators B 84, 278 (2002). [CrossRef]
  8. M. Poksinski and H. Arwin, Thin Solid Films 455-456, 716 (2004). [CrossRef]
  9. H. Arwin, in Handbook of Ellipsometry, H.G.Tompkins and E.A.Irene, eds. (Springer-Verlag, 2005), p. 799. [CrossRef]
  10. J. Mårtensson, H. Arwin, H. Nygren, and I. Lundström, J. Colloid Interface Sci. 174, 79 (1995). [CrossRef]

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