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Optics Letters

Optics Letters


  • Editor: Anthony J. Campillo
  • Vol. 32, Iss. 12 — Jun. 15, 2007
  • pp: 1680–1682

Second-harmonic-generation microscope using eight-segment polarization-mode converter to observe three-dimensional molecular orientation

Keisuke Yoshiki, Kanamaru Ryosuke, Mamoru Hashimoto, Tsutomu Araki, and Nobuyuki Hashimoto  »View Author Affiliations

Optics Letters, Vol. 32, Issue 12, pp. 1680-1682 (2007)

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We developed a compact polarization-mode converter for microscopy to control three-dimensional polarization at the focus. The converter consisted of two homogeneously aligned liquid-crystal spatial light modulators with eight independently controllable electrodes (segments), and a quarter-waveplate. The converter converted a linearly polarized beam to three polarization modes: two orthogonal linear polarizations and a pseudo-radial polarization. We applied the converter to second-harmonic-generation microscopy and demonstrated the detection of three-dimensional molecular orientation.

© 2007 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: January 12, 2007
Manuscript Accepted: March 9, 2007
Published: June 5, 2007

Virtual Issues
Vol. 2, Iss. 7 Virtual Journal for Biomedical Optics

Keisuke Yoshiki, Kanamaru Ryosuke, Mamoru Hashimoto, Nobuyuki Hashimoto, and Tsutomu Araki, "Second-harmonic-generation microscope using eight-segment polarization-mode converter to observe three-dimensional molecular orientation," Opt. Lett. 32, 1680-1682 (2007)

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