We developed a compact polarization-mode converter for microscopy to control three-dimensional polarization at the focus. The converter consisted of two homogeneously aligned liquid-crystal spatial light modulators with eight independently controllable electrodes (segments), and a quarter-waveplate. The converter converted a linearly polarized beam to three polarization modes: two orthogonal linear polarizations and a pseudo-radial polarization. We applied the converter to second-harmonic-generation microscopy and demonstrated the detection of three-dimensional molecular orientation.
© 2007 Optical Society of America
Original Manuscript: January 12, 2007
Manuscript Accepted: March 9, 2007
Published: June 5, 2007
Vol. 2, Iss. 7 Virtual Journal for Biomedical Optics
Keisuke Yoshiki, Kanamaru Ryosuke, Mamoru Hashimoto, Nobuyuki Hashimoto, and Tsutomu Araki, "Second-harmonic-generation microscope using eight-segment polarization-mode converter to observe three-dimensional molecular orientation," Opt. Lett. 32, 1680-1682 (2007)