OSA's Digital Library

Optics Letters

Optics Letters


  • Editor: Anthony J. Campillo
  • Vol. 32, Iss. 13 — Jul. 1, 2007
  • pp: 1938–1940

Lateral modulation boosts image quality in single plane illumination fluorescence microscopy

Tobias Breuninger, Klaus Greger, and Ernst H.K. Stelzer  »View Author Affiliations

Optics Letters, Vol. 32, Issue 13, pp. 1938-1940 (2007)

View Full Text Article

Enhanced HTML    Acrobat PDF (248 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



A new microscope combines optical sectioning by fluorophore excitation using a single light sheet with structured illumination. Several images with laterally intensity-modulated light sheets are recorded from scattering fluorescent specimens. By applying a simple data processing scheme, the nonmodulated volumes are identified. The blurred features become dark, and the resultant images are improved in terms of contrast and resolution. Hence, the instrument is capable of discriminating against contributions to the image that are induced by the optical properties of the specimen. The new microscope’s capabilities are demonstrated by imaging the internals of the head of an adult Drosophila melanogaster (fruit fly) expressing green fluorescent protein–labeled polycomb proteins.

© 2007 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(260.0260) Physical optics : Physical optics

ToC Category:

Original Manuscript: March 29, 2007
Manuscript Accepted: March 30, 2007
Published: June 26, 2007

Virtual Issues
Vol. 2, Iss. 8 Virtual Journal for Biomedical Optics

Tobias Breuninger, Klaus Greger, and Ernst H. K. Stelzer, "Lateral modulation boosts image quality in single plane illumination fluorescence microscopy," Opt. Lett. 32, 1938-1940 (2007)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. K. Greger, J. Swoger, and E. H. K. Stelzer, Rev. Sci. Instrum. 78, 023705 (2007). [CrossRef] [PubMed]
  2. J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, Science 305, 1007 (2004). [CrossRef] [PubMed]
  3. C. J. Engelbrecht and E. H. K. Stelzer, Opt. Lett. 31, 1477 (2006). [CrossRef] [PubMed]
  4. J. Swoger, J. Huisken, and E. H. K. Stelzer, Opt. Lett. 28, 1654 (2003). [CrossRef] [PubMed]
  5. P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, Nat. Methods 4, 311 (2007). [PubMed]
  6. M. A. Neil, R. Juskaitis, and T. Wilson, Opt. Lett. 22, 1905 (1997). [CrossRef]
  7. S. Dietzel, H. Niemann, B. Bruckner, C. Maurange, and R. Paro, Chromosoma 108, 83 (1999). [CrossRef] [PubMed]
  8. M. J. Cole, J. Siegel, S. E. D. Web, R. Jones, K. Dowling, M. J. Dayel, D. Parsons-Karavassilis, P. M. W. French, M. J. Lever, L. O. D. Sucharov, M. A. Neil, R. Juskaitis, and T. Wilson, J. Microsc. 203, 246 (2001). [CrossRef] [PubMed]
  9. L. H. Schaefer, D. Schuster, and J. Schaffer, J. Microsc. 216, 165 (2004). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited