We have developed and tested a wide-field coherent anti-Stokes Raman scattering (CARS) microscopy technique, which provides the simultaneous imaging of an extended illuminated area without scanning. This method is based on the non-phase-matching illumination of a sample and imaging of a CARS signal with a CCD camera using conventional microscope optics. We have identified a set of conditions on the illumination and imaging optics, as well as on sample preparation. Imaging of test objects proved high spatial resolution and chemical selectivity of this technique.
© 2007 Optical Society of America
Original Manuscript: February 13, 2007
Revised Manuscript: May 1, 2007
Manuscript Accepted: May 9, 2007
Published: June 26, 2007
Vol. 2, Iss. 8 Virtual Journal for Biomedical Optics
I. Toytman, K. Cohn, T. Smith, D. Simanovskii, and D. Palanker, "Wide-field coherent anti-Stokes Raman scattering microscopy with non-phase-matching illumination," Opt. Lett. 32, 1941-1943 (2007)