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Optics Letters

Optics Letters


  • Editor: Anthony J. Campillo
  • Vol. 32, Iss. 14 — Jul. 15, 2007
  • pp: 2007–2009

Aberration-free optical refocusing in high numerical aperture microscopy

Edward J. Botcherby, Rimas Juškaitis, Martin J. Booth, and Tony Wilson  »View Author Affiliations

Optics Letters, Vol. 32, Issue 14, pp. 2007-2009 (2007)

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We describe a method of optical refocusing for high numerical aperture (NA) systems that is particularly relevant for confocal and multiphoton microscopy. This method avoids the spherical aberration that is common to other optical refocusing systems. We show that aberration-free images can be obtained over an axial scan range of 70 μ m for a 1.4 NA objective lens. As refocusing is implemented remotely from the specimen, this method will enable high axial scan speeds without mechanical interference between the objective lens and the specimen.

© 2007 Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: April 5, 2007
Manuscript Accepted: May 18, 2007
Published: July 3, 2007

Virtual Issues
Vol. 2, Iss. 8 Virtual Journal for Biomedical Optics

Edward J. Botcherby, Rimas Juskaitis, Martin J. Booth, and Tony Wilson, "Aberration-free optical refocusing in high numerical aperture microscopy," Opt. Lett. 32, 2007-2009 (2007)

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