OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Anthony J. Campillo
  • Vol. 32, Iss. 16 — Aug. 15, 2007
  • pp: 2348–2350

Microscope enabling multimodality imaging, angle-resolved scattering, and scattering spectroscopy

W. J. Cottrell, J. D. Wilson, and T. H. Foster  »View Author Affiliations


Optics Letters, Vol. 32, Issue 16, pp. 2348-2350 (2007)
http://dx.doi.org/10.1364/OL.32.002348


View Full Text Article

Enhanced HTML    Acrobat PDF (247 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We present the design, construction, and initial characterization of a multifunctional imaging/scattering spectroscopy system built around a commercial inverted microscope platform. The system enables co-registered brightfield, Fourier-filtered darkfield, and fluorescence imaging; monochromatic angle-resolved scattering measurements; and white-light wavelength-resolved scattering spectroscopy from the same field of view. A fiber-based illumination system provides illumination-wavelength flexibility and a good approximation to a point source. The performance of the system in its various data acquisition modes is experimentally verified using fluorescent microspheres. This multifunctional instrument provides a platform for studies on adherent cells from which the biophysical implications of subcellular light scattering can be studied in conjunction with sensitive fluorescence-based techniques.

© 2007 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.0180) Medical optics and biotechnology : Microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(290.0290) Scattering : Scattering
(290.5820) Scattering : Scattering measurements

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: May 9, 2007
Manuscript Accepted: June 21, 2007
Published: August 2, 2007

Virtual Issues
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics

Citation
W. J. Cottrell, J. D. Wilson, and T. H. Foster, "Microscope enabling multimodality imaging, angle-resolved scattering, and scattering spectroscopy," Opt. Lett. 32, 2348-2350 (2007)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-32-16-2348


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. H. Fang, M. Ollero, E. Vitkin, L. M. Kimerer, P. B. Cipolloni, M. M. Zaman, S. D. Freedman, I. J. Bigio, I. Itzkan, E. B. Hanolon, and L. T. Perelman, IEEE J. Sel. Top. Quantum Electron. 9, 267 (2003). [CrossRef]
  2. J. R. Mourant, T. M. Johnson, S. Carpenter, A. Guerra, T. Aida, and J. P. Freyer, J. Biomed. Opt. 7, 378 (2002). [CrossRef] [PubMed]
  3. C.-C. Yu, C. Lau, J. W. Tunnell, M. Hunter, M. Kalashnikov, C. Fang-Yen, S. F. Fulghum, K. Badizadegan, R. R. Dasari, and M. S. Feld, Opt. Lett. 31, 3119 (2006). [CrossRef] [PubMed]
  4. N. N. Boustany, Y. C. Tsai, B. Pfister, W. M. Joiner, G. A. Oyler, and N. V. Thakor, Biophys. J. 87, 4163 (2004). [CrossRef] [PubMed]
  5. G. Schuele, E. Vitkin, P. Huie, C. O'Connell-Rodwell, D. Palanker, and L. T. Perelman, J. Biomed. Opt. 10, 051404 (2005). [CrossRef] [PubMed]
  6. J. D. Wilson, C. E. Bigelow, D. J. Calkins, and T. H. Foster, Biophys. J. 88, 2929 (2005). [CrossRef] [PubMed]
  7. Y. Liu, X. Li, Y. L. Kim, and V. Backman, Opt. Lett. 30, 2445 (2005). [CrossRef] [PubMed]
  8. H. Fang, L. Qiu, E. Vitkin, M. M. Zaman, C. Andersson, S. Salahuddin, L. M. Kimerer, P. B. Cipolloni, M. K. Modell, B. S. Turner, S. E. Keates, I. Bigio, I. Itzkan, S. D. Freedman, R. Bansil, E. B. Hanlon, and L. T. Perelman, Appl. Opt. 46, 1760 (2007). [CrossRef] [PubMed]
  9. M. I. Mishchenko, L. D. Travis, and A. A. Lacis, Scattering, Absorption, and Emission of Light by Small Particles (Cambridge U. Press, 2002).
  10. N. N. Boustany, S. C. Kuo, and N. V. Thakor, Opt. Lett. 26, 1063 (2001). [CrossRef]
  11. M. T. Valentine, A. K. Popp, D. A. Weitz, and P. D. Kaplan, Opt. Lett. 26, 890 (2001). [CrossRef]
  12. X. Ma, J. Q. Lu, R. S. Brock, K. M. Jacobs, P. Yang, and X.-H. Hu, Phys. Med. Biol. 48, 4165 (2003). [CrossRef]
  13. K. K.-H. Wang, J. D. Wilson, M. E. Kenney, S. Mitra, and T. H. Foster, Photochem. Photobiol. DOI: 10.1111/j.1751-1097.2007.00128.x (2007).
  14. J. D. Wilson, W. J. Cottrell, and T. H. Foster, J. Biomed. Opt. 12, 014010 (2007). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited