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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Anthony J. Campilo
  • Vol. 32, Iss. 17 — Sep. 1, 2007
  • pp: 2517–2519

Lysosome-damage-induced scattering changes coincide with release of cytochrome c

Jeremy D. Wilson, Benjamin R. Giesselman, Soumya Mitra, and Thomas H. Foster  »View Author Affiliations


Optics Letters, Vol. 32, Issue 17, pp. 2517-2519 (2007)
http://dx.doi.org/10.1364/OL.32.002517


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Abstract

Light scattering measurements made at visible wavelengths have the ability to quantify subcellular morphology. Apoptosis, or programmed cell death, is associated with distinct morphological signatures such as mitochondrial swelling and nuclear condensation as well as characteristic biochemical signaling pathways, many of which are initiated by the release of cytochrome c from the mitochondria into the cytosol. In this Letter, we examine the time course of mitochondrial morphology changes as reported by light scattering and the subcellular location of cytochrome c measured by immunofluorescence microscopy in response to intracellular cell death signaling induced by photodynamic damage to lysosomes. We report that within this system, release of cytochrome c from the mitochondria into the cytosol occurs approximately simultaneously with mitochondrial-morphology-induced light scattering changes, providing further evidence that light scattering has the potential to play an important role in future studies of cell death biology.

© 2007 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1420) Medical optics and biotechnology : Biology
(170.5180) Medical optics and biotechnology : Photodynamic therapy
(290.0290) Scattering : Scattering

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: May 2, 2007
Revised Manuscript: July 17, 2007
Manuscript Accepted: July 20, 2007
Published: August 17, 2007

Virtual Issues
Vol. 2, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Jeremy D. Wilson, Benjamin R. Giesselman, Soumya Mitra, and Thomas H. Foster, "Lysosome-damage-induced scattering changes coincide with release of cytochrome c," Opt. Lett. 32, 2517-2519 (2007)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-32-17-2517

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