We describe a technique to enhance both the weak-signal relative sensitivity and the dynamic range of a laser scanning optical microscope. The technique is based on maintaining a fixed detection power by fast feedback control of the illumination power, thereby transferring high measurement resolution to weak signals while virtually eliminating the possibility of image saturation. We analyze and demonstrate the benefits of adaptive illumination in two-photon fluorescence microscopy.
© 2007 Optical Society of America
Original Manuscript: May 16, 2007
Manuscript Accepted: August 30, 2007
Published: September 26, 2007
Vol. 2, Iss. 11 Virtual Journal for Biomedical Optics
Kengyeh K. Chu, Daryl Lim, and Jerome Mertz, "Enhanced weak-signal sensitivity in two-photon microscopy by adaptive illumination," Opt. Lett. 32, 2846-2848 (2007)