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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Anthony J. Campillo
  • Vol. 32, Iss. 19 — Oct. 1, 2007
  • pp: 2846–2848

Enhanced weak-signal sensitivity in two-photon microscopy by adaptive illumination

Kengyeh K. Chu, Daryl Lim, and Jerome Mertz  »View Author Affiliations


Optics Letters, Vol. 32, Issue 19, pp. 2846-2848 (2007)
http://dx.doi.org/10.1364/OL.32.002846


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Abstract

We describe a technique to enhance both the weak-signal relative sensitivity and the dynamic range of a laser scanning optical microscope. The technique is based on maintaining a fixed detection power by fast feedback control of the illumination power, thereby transferring high measurement resolution to weak signals while virtually eliminating the possibility of image saturation. We analyze and demonstrate the benefits of adaptive illumination in two-photon fluorescence microscopy.

© 2007 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Nonlinear Optics

History
Original Manuscript: May 16, 2007
Manuscript Accepted: August 30, 2007
Published: September 26, 2007

Virtual Issues
Vol. 2, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Kengyeh K. Chu, Daryl Lim, and Jerome Mertz, "Enhanced weak-signal sensitivity in two-photon microscopy by adaptive illumination," Opt. Lett. 32, 2846-2848 (2007)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-32-19-2846


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References

  1. J. B. Pawley, Handbook of Biological Confocal Microscopy (Springer, 2006). [CrossRef]
  2. W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990). [CrossRef] [PubMed]
  3. E. Meyer, H. J. Hug, and R. Bennewitz, Scanning Probe Microscopy: the Lab on a Tip (Springer, 2003).
  4. R. A. Hoebe, C. H. Van Oven, T. W. J. Gadella Jr., P. B. Dhonukshe, C. J. F. Van Noorden, and E. M. M. Manders, Nat. Biotechnol. 25, 249 (2007). [CrossRef] [PubMed]
  5. G. H. Patterson and D. W. Piston, Biophys. J. 78, 2159 (2000). [CrossRef] [PubMed]

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