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Optics Letters

Optics Letters


  • Editor: Anthony J. Campillo
  • Vol. 32, Iss. 2 — Jan. 15, 2007
  • pp: 145–147

Feedback localization of freely diffusing fluorescent particles near the optical shot-noise limit

Andrew J. Berglund, Kevin McHale, and Hideo Mabuchi  »View Author Affiliations

Optics Letters, Vol. 32, Issue 2, pp. 145-147 (2007)

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We report near-optimal tracking of freely diffusing fluorescent particles in a quasi-two-dimensional geometry via photon counting and real-time feedback. We present a quantitative statistical model of our feedback network and find excellent agreement with the experiment. We monitor the motion of a single fluorescent particle with a sensitivity of 15 nm Hz while collecting fewer than 5000 fluorescence photons s . Fluorescent microspheres (diffusion coefficient 1.3 μ m 2 s ) are tracked with a root-mean-square tracking error of 170 nm , within a factor of 2 of the theoretical limit set by photon counting shot noise.

© 2006 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:

Original Manuscript: August 7, 2006
Revised Manuscript: October 10, 2006
Manuscript Accepted: October 12, 2006
Published: December 23, 2006

Virtual Issues
Vol. 2, Iss. 2 Virtual Journal for Biomedical Optics

Andrew J. Berglund, Kevin McHale, and Hideo Mabuchi, "Feedback localization of freely diffusing fluorescent particles near the optical shot-noise limit," Opt. Lett. 32, 145-147 (2007)

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  1. T. Ha, D. S. Chemla, T. Enderle, and S. Weiss, Appl. Phys. Lett. 70, 782 (1997). [CrossRef]
  2. J. Enderlein, Appl. Phys. B 71, 773 (2000). [CrossRef]
  3. A. J. Berglund and H. Mabuchi, Appl. Phys. B 78, 653 (2004). [CrossRef]
  4. V. Levi, Q. Ruan, and E. Gratton, Biophys. J. 88, 2919 (2005). [CrossRef] [PubMed]
  5. A. J. Berglund and H. Mabuchi, Opt. Express 13, 8069 (2005). [CrossRef] [PubMed]
  6. S. B. Andersson, Appl. Phys. B 80, 809 (2005). [CrossRef]
  7. A. E. Cohen and W. E. Moerner, Proc. Natl. Acad. Sci. U.S.A. 103, 4362 (2006). [CrossRef] [PubMed]
  8. A. J. Berglund and H. Mabuchi, Appl. Phys. B 83, 127 (2006). [CrossRef]
  9. C. W. Gardiner, Handbook of Stochastic Methods for Physics, Chemistry and the Natural Sciences, 2nd ed. (Springer-Verlag, 1985).
  10. N. G. Van Kampen, Stochastic Processes in Physics and Chemistry (Elsevier Science, 2001).
  11. O. L. R. Jacobs, Introduction to Control Theory, 2nd ed. (Oxford U. Press, 1996).

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