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Optics Letters

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  • Editor: Anthony J. Campillo
  • Vol. 32, Iss. 2 — Jan. 15, 2007
  • pp: 145–147

Feedback localization of freely diffusing fluorescent particles near the optical shot-noise limit

Andrew J. Berglund, Kevin McHale, and Hideo Mabuchi  »View Author Affiliations


Optics Letters, Vol. 32, Issue 2, pp. 145-147 (2007)
http://dx.doi.org/10.1364/OL.32.000145


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Abstract

We report near-optimal tracking of freely diffusing fluorescent particles in a quasi-two-dimensional geometry via photon counting and real-time feedback. We present a quantitative statistical model of our feedback network and find excellent agreement with the experiment. We monitor the motion of a single fluorescent particle with a sensitivity of 15 nm Hz while collecting fewer than 5000 fluorescence photons s . Fluorescent microspheres (diffusion coefficient 1.3 μ m 2 s ) are tracked with a root-mean-square tracking error of 170 nm , within a factor of 2 of the theoretical limit set by photon counting shot noise.

© 2006 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:
Microscopy

History
Original Manuscript: August 7, 2006
Revised Manuscript: October 10, 2006
Manuscript Accepted: October 12, 2006
Published: December 23, 2006

Virtual Issues
Vol. 2, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Andrew J. Berglund, Kevin McHale, and Hideo Mabuchi, "Feedback localization of freely diffusing fluorescent particles near the optical shot-noise limit," Opt. Lett. 32, 145-147 (2007)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-32-2-145


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References

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