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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Anthony J. Campillo
  • Vol. 32, Iss. 3 — Feb. 1, 2007
  • pp: 259–261

4Pi microscopy with linear fluorescence excitation

Marion C. Lang, Johann Engelhardt, and Stefan W. Hell  »View Author Affiliations


Optics Letters, Vol. 32, Issue 3, pp. 259-261 (2007)
http://dx.doi.org/10.1364/OL.32.000259


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Abstract

Practical 4Pi microscopy has so far exclusively relied on multiphoton excitation of fluorescence, because the nonlinear suppression of contributions from higher-order sidelobes was mandatory for unambiguous axial superresolution. We show that novel lenses of 74 ° semiaperture angle enable biological 4Pi microscopy with regular one-photon fluorescence excitation, thus increasing the signal and reducing system complexity and cost. An axial resolution of 95 nm , corresponding to a more than fourfold improvement over confocal microscopy, is verified in the imaging of microtubules in mammalian cells.

© 2007 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Microscopy

History
Original Manuscript: September 1, 2006
Revised Manuscript: October 21, 2006
Manuscript Accepted: October 26, 2006
Published: January 12, 2007

Virtual Issues
Vol. 2, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Marion C. Lang, Johann Engelhardt, and Stefan W. Hell, "4Pi microscopy with linear fluorescence excitation," Opt. Lett. 32, 259-261 (2007)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-32-3-259


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References

  1. S. Hell and E. H. K. Stelzer, J. Opt. Soc. Am. A 9, 2159 (1992). [CrossRef]
  2. S. W. Hell, "Double-scanning confocal microscope," European Patent 0491289 (1990).
  3. H. Gugel, J. Bewersdorf, S. Jakobs, J. Engelhardt, R. Storz, and S. W. Hell, Biophys. J. 87, 4146 (2004). [CrossRef] [PubMed]
  4. M. G. L. Gustafsson, D. A. Agard, and J. W. Sedat, in Proc. SPIE 2412, 147 (1995). [CrossRef]
  5. M. G. L. Gustafsson, D. A. Agard, and J. W. Sedat, J. Microsc. 195, 10 (1999). [CrossRef] [PubMed]
  6. J. Bewersdorf, R. Schmidt, and S. W. Hell, J. Microsc. 222, 105 (2006). [CrossRef] [PubMed]
  7. M. Nagorni and S. W. Hell, J. Opt. Soc. Am. A 18, 36 (2001). [CrossRef]
  8. S. W. Hell and E. H. K. Stelzer, Opt. Commun. 93, 277 (1992). [CrossRef]
  9. S. W. Hell, in Topics in Fluorescence Spectroscopy, J.R.Lakowicz, ed. (Plenum, 1997), p. 361.
  10. M. Martínez-Corral, Micron 34, 319 (2003). [CrossRef] [PubMed]
  11. M. Martínez-Corral, A. Pons, and M. T. Caballero, J. Opt. Soc. Am. A 19, 1532 (2002). [CrossRef]
  12. B. Richards and E. Wolf, Proc. R. Soc. London, Ser. A 253, 358 (1959). [CrossRef]
  13. A. N. Tikhonov and V. Y. Arsenin, Solutions of Ill-Posed Problems (Wiley, 1977).
  14. W. H. Richardson, J. Opt. Soc. Am. 62, 55 (1972). [CrossRef]

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