Spectral-domain optical coherence phase and multiphoton microscopy
Optics Letters, Vol. 32, Issue 6, pp. 623-625 (2007)
http://dx.doi.org/10.1364/OL.32.000623
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Abstract
We describe simultaneous quantitative phase contrast and multiphoton fluorescence imaging by combined spectral-domain optical coherence phase and multiphoton microscopy. The instrument employs two light sources for efficient optical coherence microscopic and multiphoton imaging and can generate structural and functional images of transparent specimens in the epidirection. Phase contrast imaging exhibits spatial and temporal phase stability in the subnanometer range. We also demonstrate the visualization of actin filaments in a fixed cell specimen, which is confirmed by simultaneous multiphoton fluorescence imaging.
© 2007 Optical Society of America
OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.4500) Imaging systems : Optical coherence tomography
(180.2520) Microscopy : Fluorescence microscopy
(180.3170) Microscopy : Interference microscopy
ToC Category:
Microscopy
History
Original Manuscript: November 13, 2006
Manuscript Accepted: December 12, 2006
Published: February 15, 2007
Virtual Issues
Vol. 2, Iss. 4 Virtual Journal for Biomedical Optics
Citation
Chulmin Joo, Ki Hean Kim, and Johannes F. de Boer, "Spectral-domain optical coherence phase and multiphoton microscopy," Opt. Lett. 32, 623-625 (2007)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-32-6-623
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