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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Anthony J. Campillo
  • Vol. 32, Iss. 6 — Mar. 15, 2007
  • pp: 623–625

Spectral-domain optical coherence phase and multiphoton microscopy

Chulmin Joo, Ki Hean Kim, and Johannes F. de Boer  »View Author Affiliations


Optics Letters, Vol. 32, Issue 6, pp. 623-625 (2007)
http://dx.doi.org/10.1364/OL.32.000623


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Abstract

We describe simultaneous quantitative phase contrast and multiphoton fluorescence imaging by combined spectral-domain optical coherence phase and multiphoton microscopy. The instrument employs two light sources for efficient optical coherence microscopic and multiphoton imaging and can generate structural and functional images of transparent specimens in the epidirection. Phase contrast imaging exhibits spatial and temporal phase stability in the subnanometer range. We also demonstrate the visualization of actin filaments in a fixed cell specimen, which is confirmed by simultaneous multiphoton fluorescence imaging.

© 2007 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.4500) Imaging systems : Optical coherence tomography
(180.2520) Microscopy : Fluorescence microscopy
(180.3170) Microscopy : Interference microscopy

ToC Category:
Microscopy

History
Original Manuscript: November 13, 2006
Manuscript Accepted: December 12, 2006
Published: February 15, 2007

Virtual Issues
Vol. 2, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Chulmin Joo, Ki Hean Kim, and Johannes F. de Boer, "Spectral-domain optical coherence phase and multiphoton microscopy," Opt. Lett. 32, 623-625 (2007)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-32-6-623

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