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Optics Letters

Optics Letters


  • Editor: Anthony J. Campillo
  • Vol. 32, Iss. 6 — Mar. 15, 2007
  • pp: 659–661

In vivo fluorescent imaging of the mouse retina using adaptive optics

David P. Biss, Daniel Sumorok, Stephen A. Burns, Robert H. Webb, Yaopeng Zhou, Thomas G. Bifano, Daniel Côté, Israel Veilleux, Parisa Zamiri, and Charles P. Lin  »View Author Affiliations

Optics Letters, Vol. 32, Issue 6, pp. 659-661 (2007)

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In vivo imaging of the mouse retina using visible and near infrared wavelengths does not achieve diffraction-limited resolution due to wavefront aberrations induced by the eye. Considering the pupil size and axial dimension of the eye, it is expected that unaberrated imaging of the retina would have a transverse resolution of 2 μ m . Higher-order aberrations in retinal imaging of human can be compensated for by using adaptive optics. We demonstrate an adaptive optics system for in vivo imaging of fluorescent structures in the retina of a mouse, using a microelectromechanical system membrane mirror and a Shack–Hartmann wavefront sensor that detects fluorescent wavefront.

© 2007 Optical Society of America

OCIS Codes
(010.1080) Atmospheric and oceanic optics : Active or adaptive optics
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: September 14, 2006
Revised Manuscript: December 1, 2006
Manuscript Accepted: December 12, 2006
Published: February 15, 2007

Virtual Issues
Vol. 2, Iss. 4 Virtual Journal for Biomedical Optics

David P. Biss, Daniel Sumorok, Stephen A. Burns, Robert H. Webb, Yaopeng Zhou, Thomas G. Bifano, Daniel Côté, Israel Veilleux, Parisa Zamiri, and Charles P. Lin, "In vivo fluorescent imaging of the mouse retina using adaptive optics," Opt. Lett. 32, 659-661 (2007)

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  1. M. Paques, M. Simonutti, M. J. Roux, S. Picaud, E. Levavassuer, C. Bellman, and J.-A. Sahel, Vision Res. 46, 1336 (2006). [CrossRef]
  2. T. Higashide, I. Kawaguchi, S. Ohkubo, H. Takeda, and K. Sugiyama, Invest. Opthalmol. Visual Sci. 47, 2943 (2006). [CrossRef]
  3. E. García de la Cera, G. Rodríguez, L. Llorente, F. Schaeffel, and S. Marcos, Vision Res. 46, 2546 (2006). [CrossRef]
  4. J. Liang, D. R. Williams, and D. Miller, J. Opt. Soc. Am. A 14, 2884 (1997). [CrossRef]
  5. A. Roorda and D. R. Williams, Nature 397, 520 (1999). [CrossRef] [PubMed]
  6. N. Doble, G. Yoon, L. Chen, P. Bierden, B. Singer, S. Olivier, and D. R. Williams, Opt. Lett. 27, 1537 (2002). [CrossRef]
  7. A. Roorda, F. Romero-Borja, W. J. Donnelly III, H. Queener, T. J. Herbert, and M. C. W. Campbell, Opt. Express 10, 405 (2006).
  8. Y. Zhang, S. Poonja, and A. Roorda, Opt. Lett. 31, 1268 (2006). [CrossRef] [PubMed]
  9. D. X. Hammer, R. D. Ferguson, C. E. Bigelow, N. V. Iftimia, T. E. Ustun, and S. A. Burns, Opt. Express 14, 3354 (2006). [CrossRef] [PubMed]
  10. D. C. Gray, W. Merigan, J. I. Wolfing, B. P. Gee, J. Porter, A. Dubra, T. H. Twietmeyer, K. Ahamd, R. Tumbar, E. Reinholz, and D. R. Williams, Opt. Express 14, 7144 (2006). [CrossRef] [PubMed]
  11. F. C. Delori, R. H. Webb, and D. M. Sliney, 'Maximum permissible exposures for ocular safety (ANSI 2000), with emphasis on ophthalmic devices,' J. Opt. Soc. A (to be published).
  12. W. S. Rasband, 'ImageJ' National Institutes of Health, Bethesda, Maryland, USA, http://rsb.info.nih.gov/ij/, 1997-2006.
  13. L. N. Thibos, R. A. Applegate, J. T. Schwiegerling, R. H. Webb, and V. S. T. Members, in Vision Science and its Applications, V.Lakshminarayanan, ed., Vol. 35 of OSA Trends in Optics and Photonics Series (Optical Society of America, 2000), pp. 232-244.
  14. R. H. Webb, M. J. Albanese, Y. Zhou, T. Bifano, and S. A. Burns, Appl. Opt. 43, 5330 (2004). [CrossRef] [PubMed]

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