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Optics Letters

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  • Editor: Alan E. Willner
  • Vol. 33, Iss. 10 — May. 15, 2008
  • pp: 1038–1040

Microvascular oxygen quantification using two-photon microscopy

Arnold D. Estrada, Adrien Ponticorvo, Tim N. Ford, and Andrew K. Dunn  »View Author Affiliations


Optics Letters, Vol. 33, Issue 10, pp. 1038-1040 (2008)
http://dx.doi.org/10.1364/OL.33.001038


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Abstract

An instrument is demonstrated that is capable of three-dimensional (3D) vasculature imaging and pO 2 quantification with high spatial resolution. The instrument combines two-photon (2P) microscopy with phosphorescence quenching to measure pO 2 . The instrument was demonstrated by performing depth-resolved microvascular pO 2 measurements of rat cortical vessels down to 120 μ m below the surface. 2P excitation of porphyrin was confirmed, and measured pO 2 values were consistent with previously published data for normoxic and hyperoxic conditions. The ability to perform 3D pO 2 measurements using optical techniques will allow researchers to overcome existing limitations imposed by polarographic electrodes, magnetic resonance techniques, and surface-only pO 2 measurement techniques.

© 2008 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.1460) Medical optics and biotechnology : Blood gas monitoring
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: November 29, 2007
Revised Manuscript: March 20, 2008
Manuscript Accepted: March 21, 2008
Published: May 2, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Arnold D. Estrada, Adrien Ponticorvo, Tim N. Ford, and Andrew K. Dunn, "Microvascular oxygen quantification using two-photon microscopy," Opt. Lett. 33, 1038-1040 (2008)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-33-10-1038


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References

  1. J. M. Vanderkooi and D. F. Wilson, Adv. Exp. Med. Biol. 200, 189 (1986). [PubMed]
  2. J. M. Vanderkooi, G. Maniara, and T. J. Green, J. Biol. Chem. 262, 5476 (1987). [PubMed]
  3. R. P. Briñas, T. Troxler, and R. M. Hochstrasser, J. Am. Chem. Soc. 127, 11851 (2005). [CrossRef] [PubMed]
  4. M. Shibata, S. Ichioka, and J. Ando, J. Appl. Physiol. 91, 321 (2001). [PubMed]
  5. R. D. Shonat and A. C. Kight, Ann. Biomed. Eng. 31, 1084 (2003). [CrossRef] [PubMed]
  6. L. Zheng, A. S. Golub, and R. N. Pittman, Am. J. Physiol. 271, H365 (1996). [PubMed]
  7. R. D. Shonat, K. N. Richmond, and P. C. Johnson, Rev. Sci. Instrum. 66, 5075 (1995). [CrossRef]
  8. E. G. Mik, T. G. V. Leeuwen, and N. J. Raat, J. Appl. Physiol. 97, 1962 (2004). [CrossRef] [PubMed]
  9. D. Kleinfeld and W. Denk, in Imaging Neurons (Cold Spring Harbor Laboratory Press, 1999).
  10. I. Dunphy, S. A. Vinogradov, and D. F. Wilson, Anal. Biochem. 310, 191 (2002). [CrossRef] [PubMed]
  11. R. D. Shonat, E. S. Wachman, and W. Niu, Biophys. J. 73, 1223 (1997). [CrossRef] [PubMed]

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