OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 33, Iss. 10 — May. 15, 2008
  • pp: 1038–1040

Microvascular oxygen quantification using two-photon microscopy

Arnold D. Estrada, Adrien Ponticorvo, Tim N. Ford, and Andrew K. Dunn  »View Author Affiliations


Optics Letters, Vol. 33, Issue 10, pp. 1038-1040 (2008)
http://dx.doi.org/10.1364/OL.33.001038


View Full Text Article

Enhanced HTML    Acrobat PDF (293 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

An instrument is demonstrated that is capable of three-dimensional (3D) vasculature imaging and pO 2 quantification with high spatial resolution. The instrument combines two-photon (2P) microscopy with phosphorescence quenching to measure pO 2 . The instrument was demonstrated by performing depth-resolved microvascular pO 2 measurements of rat cortical vessels down to 120 μ m below the surface. 2P excitation of porphyrin was confirmed, and measured pO 2 values were consistent with previously published data for normoxic and hyperoxic conditions. The ability to perform 3D pO 2 measurements using optical techniques will allow researchers to overcome existing limitations imposed by polarographic electrodes, magnetic resonance techniques, and surface-only pO 2 measurement techniques.

© 2008 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.1460) Medical optics and biotechnology : Blood gas monitoring
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: November 29, 2007
Revised Manuscript: March 20, 2008
Manuscript Accepted: March 21, 2008
Published: May 2, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Arnold D. Estrada, Adrien Ponticorvo, Tim N. Ford, and Andrew K. Dunn, "Microvascular oxygen quantification using two-photon microscopy," Opt. Lett. 33, 1038-1040 (2008)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-33-10-1038

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited