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Optics Letters


  • Editor: Alan E. Willner
  • Vol. 33, Iss. 12 — Jun. 15, 2008
  • pp: 1333–1335

Fast handheld two-photon fluorescence microendoscope with a 475 μ m × 475 μ m field of view for in vivo imaging

Hongchun Bao, John Allen, Robert Pattie, Rod Vance, and Min Gu  »View Author Affiliations

Optics Letters, Vol. 33, Issue 12, pp. 1333-1335 (2008)

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A fast handheld two-photon fiber-optic fluorescence endoscope for three-dimensional (3D) in vivo cellular imaging is developed. The compact handheld probe of the two-photon endoscope can simply be placed into contact with the target tissue to reveal clear 3D surface and subsurface histological images without biopsy. The new system has, to the best of our knowledge, the largest field of view (FOV) of 475 μ m × 475 μ m and a 3D imaging volume larger than 475 μ m × 475 μ m × 250 μ m . A real-time two-photon fluorescence image is displayed at 0.4 mm 2 s . The lateral and axial resolutions of the two-photon fluorescence endoscope are better than 1 and 14.5 μ m , respectively.

© 2008 Optical Society of America

OCIS Codes
(110.2350) Imaging systems : Fiber optics imaging
(180.6900) Microscopy : Three-dimensional microscopy
(190.4370) Nonlinear optics : Nonlinear optics, fibers

ToC Category:

Original Manuscript: February 13, 2008
Revised Manuscript: April 28, 2008
Manuscript Accepted: May 5, 2008
Published: June 12, 2008

Virtual Issues
Vol. 3, Iss. 7 Virtual Journal for Biomedical Optics

Hongchun Bao, John Allen, Robert Pattie, Rod Vance, and Min Gu, "Fast handheld two-photon fluorescence microendoscope with a 475 μm × 475 μm field of view for in vivo imaging," Opt. Lett. 33, 1333-1335 (2008)

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  1. W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990). [CrossRef] [PubMed]
  2. B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, Nat. Methods 12, 941 (2005). [CrossRef]
  3. L. Fu and M. Gu, J. Microsc. 226, 195 (2007). [CrossRef] [PubMed]
  4. F. Helmchen, M. S. Fee, D. W. Tank, and W. Denk, Neuron 31, 903 (2001). [CrossRef] [PubMed]
  5. D. Bird and M. Gu, Opt. Lett. 27, 1031 (2002). [CrossRef]
  6. D. Bird and M. Gu, Opt. Lett. 28, 1552 (2003). [CrossRef] [PubMed]
  7. L. Fu, X. Gan, and M. Gu, Opt. Lett. 30, 385 (2005). [CrossRef] [PubMed]
  8. L. Fu, X. Gan, and M. Gu, Opt. Express 13, 5528 (2005). [CrossRef] [PubMed]
  9. L. Fu, A. Jain, C. Cranfield, H. Xie, and M. Gu, J. Biomed. Opt. 12, 040501 (2007). [CrossRef] [PubMed]
  10. L. Fu and M. Gu, Opt. Lett. 31, 1471 (2006). [CrossRef] [PubMed]
  11. W. Piyawattanametha, R. P. J. Barretto, T. H. Ko, B. A. Flusberg, E. D. Cocker, H. Ra, D. Lee, and O. Solgaard, Opt. Lett. 31, 2018 (2006). [CrossRef] [PubMed]
  12. B. A. Flusberg, J. C. Jung, E. D. Cocker, E. P. Anderson, and M. J. Schnitzer, Opt. Lett. 30, 2272 (2005). [CrossRef] [PubMed]
  13. M. T. Myaing, D. J. MacDonald, and X. Li, Opt. Lett. 31, 1076 (2006). [CrossRef] [PubMed]
  14. S. W. Clark, F. O. Ilday, and F. W. Wise, Opt. Lett. 26, 1320 (2001). [CrossRef]
  15. M. Lelek, E. Suran, F. Louradour, A. Barthelemy, B. Viellerobe, and F. Lacombe, Opt. Express 15, 10154 (2007). [CrossRef] [PubMed]
  16. M. Goetz, C. Fottner, E. Schirrmacher, P. Delaney, S. Gregor, C. Schneider, D. Strand, S. Kanzler, B. Memadathil, E. Weyand, M. Holtmann, R. Schirrmacher, M. M. Weber, M. Anlauf, G. Klöppel, M. Vieth, P. R. Galle, P. Bartenstein, M. F. Neurath, and R. Kiesslich, Endoscopy 39, 350 (2007). [CrossRef] [PubMed]
  17. M. Gu, Advanced Optical Imaging Theory, Springer Series in Optical Sciences 75 (Springer-Verlag, 2000).

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