Field-based angle-resolved light-scattering study of single live cells
Optics Letters, Vol. 33, Issue 14, pp. 1596-1598 (2008)
http://dx.doi.org/10.1364/OL.33.001596
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Abstract
We perform field-based angle-resolved light-scattering measurements from single live cells. We use a laser interferometer to acquire phase and amplitude images of cells at the image plane. The angular scattering spectrum is calculated from the Fourier transform of the field transmitted through the cells. A concurrent 3D refractive index distribution of the same cells is measured using tomographic phase microscopy. By measuring transient increases in light scattering by single cells during exposure to acetic acid, we correlate the scattering properties of single cells with their refractive index distributions and show that results are in good agreement with a model based on the Born approximation.
© 2008 Optical Society of America
OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.3880) Medical optics and biotechnology : Medical and biological imaging
ToC Category:
Instrumentation, Measurement, and Metrology
History
Original Manuscript: April 3, 2008
Revised Manuscript: May 23, 2008
Manuscript Accepted: May 26, 2008
Published: July 14, 2008
Virtual Issues
Vol. 3, Iss. 8 Virtual Journal for Biomedical Optics
Citation
Wonshik Choi, Chung-Chieh Yu, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld, "Field-based angle-resolved light-scattering study of single live cells," Opt. Lett. 33, 1596-1598 (2008)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-33-14-1596
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