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Optics Letters

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  • Editor: Alan E. Willner
  • Vol. 33, Iss. 14 — Jul. 15, 2008
  • pp: 1611–1613

Sub- 100 nm IR spectromicroscopy of living cells

C. Mayet, A. Dazzi, R. Prazeres, F. Allot, F. Glotin, and J. M. Ortega  »View Author Affiliations


Optics Letters, Vol. 33, Issue 14, pp. 1611-1613 (2008)
http://dx.doi.org/10.1364/OL.33.001611


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Abstract

We have performed IR spectromicroscopy of cells immersed in liquid water, with a lateral resolution better than 100 nm . Here, we use the motion of an atomic force microscope tip, probing the local transient deformation induced by an IR pulsed laser tuned at a sample absorbing wavelength. By Fourier analysis of the vibration of the cantilever tip, we can discriminate frequencies that are characteristic of the object, thus eliminating the influence of the water absorption. This opens the door of chemical imaging of living species in vivo, with spatial resolution of the order of the size of cell components.

© 2008 Optical Society of America

OCIS Codes
(100.2960) Image processing : Image analysis
(180.5810) Microscopy : Scanning microscopy

ToC Category:
Microscopy

History
Original Manuscript: February 26, 2008
Revised Manuscript: June 12, 2008
Manuscript Accepted: June 17, 2008
Published: July 14, 2008

Virtual Issues
Vol. 3, Iss. 8 Virtual Journal for Biomedical Optics

Citation
C. Mayet, A. Dazzi, R. Prazeres, F. Allot, F. Glotin, and J. M. Ortega, "Sub-100 nm IR spectromicroscopy of living cells," Opt. Lett. 33, 1611-1613 (2008)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-33-14-1611


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References

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