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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 33, Iss. 14 — Jul. 15, 2008
  • pp: 1617–1619

Axial coding in full-field microscopy using three-dimensional structured illumination implemented with no moving parts

Pedro FelipeGardeazábal Rodríguez, Eduardo Sepulveda, Benoit Dubertret, and Vincent Loriette  »View Author Affiliations

Optics Letters, Vol. 33, Issue 14, pp. 1617-1619 (2008)

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We report a simple optical setup to produce both axial and lateral structured illumination through a single objective lens. With a minimum of six full-field images obtained without moving either the sample or the microscope objective, 100 nm diameter fluorescent beads can be localized axially with an accuracy of 50 nm in a 1.76 - μ m -thick layer. We show that this axial localization improvement can easily be combined with classical lateral structured illumination, so that lateral resolution enhancement by a factor of 2 is maintained.

© 2008 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: April 1, 2008
Manuscript Accepted: May 23, 2008
Published: July 14, 2008

Virtual Issues
Vol. 3, Iss. 8 Virtual Journal for Biomedical Optics

Pedro Felipe Gardeazábal Rodríguez, Eduardo Sepulveda, Benoit Dubertret, and Vincent Loriette, "Axial coding in full-field microscopy using three-dimensional structured illumination implemented with no moving parts," Opt. Lett. 33, 1617-1619 (2008)

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