OSA's Digital Library

Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 33, Iss. 14 — Jul. 15, 2008
  • pp: 1626–1628

Extended focused imaging of a microparticle field with digital holographic microscopy

Maciej Antkowiak, Natacha Callens, Catherine Yourassowsky, and Frank Dubois  »View Author Affiliations

Optics Letters, Vol. 33, Issue 14, pp. 1626-1628 (2008)

View Full Text Article

Enhanced HTML    Acrobat PDF (242 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We present a numerical technique for extended focused imaging and three-dimensional analysis of a microparticle field observed in a digital holographic microscope working in transmission. The three-dimensional localization of objects is performed using the local focus plane determination method based on the integrated amplitude modulus. We apply the refocusing criterion locally for each pixel, using small overlapping windows, to obtain the depth map and a synthetic image in which all objects are refocused independent from their refocusing distance. A successful application of this technique in the analysis of the microgravity particle flow experiment is presented.

© 2008 Optical Society of America

OCIS Codes
(090.1760) Holography : Computer holography
(090.2880) Holography : Holographic interferometry
(100.0100) Image processing : Image processing
(180.3170) Microscopy : Interference microscopy

ToC Category:

Original Manuscript: May 8, 2008
Manuscript Accepted: June 4, 2008
Published: July 14, 2008

Virtual Issues
Vol. 3, Iss. 8 Virtual Journal for Biomedical Optics

Maciej Antkowiak, Natacha Callens, Catherine Yourassowsky, and Frank Dubois, "Extended focused imaging of a microparticle field with digital holographic microscopy," Opt. Lett. 33, 1626-1628 (2008)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. T. Kreis, J. Opt. Soc. Am. A 3, 3 (1986). [CrossRef]
  2. E. Cuche, F. Bevilacqua, and C. Depeursinge, Opt. Lett. 24, 291 (1999). [CrossRef]
  3. F. Dubois, N. Callens, C. Yourassowsky, M. Hoyos, P. Kurowski, and O. Monnom, Appl. Opt. 45, 864 (2006). [CrossRef] [PubMed]
  4. L. Yu and L. Cai, J. Opt. Soc. Am. A 18, 1033 (2001). [CrossRef]
  5. J. Gillespie and R. King, Pattern Recogn. Lett. 9, 19 (1989). [CrossRef]
  6. L. Ma, H. Wang, Y. Li, and H. Jin, J. Opt. A Pure Appl. Opt. 6, 396 (2004). [CrossRef]
  7. W. Li, N. Loomis, Q. Hu, and C. Davis, J. Opt. Soc. Am. A 24, 3054 (2007). [CrossRef]
  8. M. Liebling and M. Unser, J. Opt. Soc. Am. A 21, 2424 (2004). [CrossRef]
  9. C. McElhinney, J. McDonald, A. Castro, Y. Frauel, B. Javidi, and T. Naughton, Opt. Lett. 32, 1229 (2007). [CrossRef] [PubMed]
  10. C. McElhinney, B. Hennelly, and T. Naughton, Appl. Opt. 47, D71 (2008). [CrossRef] [PubMed]
  11. P. Ferraro, S. Grilli, D. Alfieri, S. De Nicola, A. Finizio, G. Pierattini, B. Javidi, G. Coppola, and V. Striano, Opt. Express 13, 6738 (2005). [CrossRef] [PubMed]
  12. C. Buraga-Lefebvre, S. Coëtmellec, D. Lebrun, and C. Özkul, Opt. Lasers Eng. 33, 409 (2000). [CrossRef]
  13. D. Lebrun, A. Benkouider, S. Coëtmellec, and M. Malek, Opt. Express 11, 224 (2003). [CrossRef] [PubMed]
  14. Y. Park, G. Popescu, K. Badizadegan, R. Dasari, and M. Feld, Opt. Lett. 32, 811 (2007). [CrossRef] [PubMed]
  15. J. Sheng, E. Malkiel, and J. Katz, Appl. Opt. 45, 3893 (2006). [CrossRef] [PubMed]
  16. B. Javidi, S. Yeom, I. Moon, and M. Daneshpanah, Opt. Express 14, 3806 (2006). [CrossRef] [PubMed]
  17. F. Dubois, C. Schockaert, N. Callens, and C. Yourassowsky, Opt. Express 14, 5895 (2006). [CrossRef] [PubMed]
  18. C. Schockaert, Ph.D. dissertation “Three-dimensional object analysis and tracking by digital holography microscopy” (Université Libre de Bruxelles, 2006).
  19. N. Callens, Ph.D. dissertation, “Développment, étude expérimentale et visualisation par holographie digitale de mini-séparateurs fluidiques (STEP-SPLITT) en vue de la séparation d'objects de taille micrométrique” (Université Paris VI and Université Libre de Bruxelles, 2005).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited