We present a numerical technique for extended focused imaging and three-dimensional analysis of a microparticle field observed in a digital holographic microscope working in transmission. The three-dimensional localization of objects is performed using the local focus plane determination method based on the integrated amplitude modulus. We apply the refocusing criterion locally for each pixel, using small overlapping windows, to obtain the depth map and a synthetic image in which all objects are refocused independent from their refocusing distance. A successful application of this technique in the analysis of the microgravity particle flow experiment is presented.
© 2008 Optical Society of America
Original Manuscript: May 8, 2008
Manuscript Accepted: June 4, 2008
Published: July 14, 2008
Vol. 3, Iss. 8 Virtual Journal for Biomedical Optics
Maciej Antkowiak, Natacha Callens, Catherine Yourassowsky, and Frank Dubois, "Extended focused imaging of a microparticle field with digital holographic microscopy," Opt. Lett. 33, 1626-1628 (2008)