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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 33, Iss. 14 — Jul. 15, 2008
  • pp: 1626–1628

Extended focused imaging of a microparticle field with digital holographic microscopy

Maciej Antkowiak, Natacha Callens, Catherine Yourassowsky, and Frank Dubois  »View Author Affiliations


Optics Letters, Vol. 33, Issue 14, pp. 1626-1628 (2008)
http://dx.doi.org/10.1364/OL.33.001626


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Abstract

We present a numerical technique for extended focused imaging and three-dimensional analysis of a microparticle field observed in a digital holographic microscope working in transmission. The three-dimensional localization of objects is performed using the local focus plane determination method based on the integrated amplitude modulus. We apply the refocusing criterion locally for each pixel, using small overlapping windows, to obtain the depth map and a synthetic image in which all objects are refocused independent from their refocusing distance. A successful application of this technique in the analysis of the microgravity particle flow experiment is presented.

© 2008 Optical Society of America

OCIS Codes
(090.1760) Holography : Computer holography
(090.2880) Holography : Holographic interferometry
(100.0100) Image processing : Image processing
(180.3170) Microscopy : Interference microscopy

ToC Category:
Holography

History
Original Manuscript: May 8, 2008
Manuscript Accepted: June 4, 2008
Published: July 14, 2008

Virtual Issues
Vol. 3, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Maciej Antkowiak, Natacha Callens, Catherine Yourassowsky, and Frank Dubois, "Extended focused imaging of a microparticle field with digital holographic microscopy," Opt. Lett. 33, 1626-1628 (2008)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-33-14-1626


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